网纹藤壶壳板矿化结构及其碳酸钙含量测定  

Microstructure and content of biomineralizing calcium carbonate in the shell of Balanus reticulates Utinomi

作　　者：赖水发[1] 陈新[1,2] 彭明栋[1] 张彬山[3] 王芳[1] 张哲进[1] 唐敏[1,2] 

机构地区：[1]海南大学材料与化工学院,海南海口570228 [2]海南大学热带岛屿资源先进材料教育部重点实验室,海南海口570228 [3]海南大学海洋学院,海南海口570228

出　　处：《海洋科学》2013年第6期32-36,共5页Marine Sciences

基　　金：国家自然科学基金项目(31160098);海南省自然科学基金项目(313104);海南大学博士科研启动基金项目(kyqd1046)

摘　　要：采用X射线衍射技术,初步研究了网纹藤壶(Balanus reticulates Utinomi)壳板矿化结构特征,并采用钙黄绿素法、钙-羧酸指示剂法和铬黑T指示剂三种方法测量了来自不同海域藤壶壳板的碳酸钙含量。实验发现,网纹藤壶壳板中的矿化组织主要由方解石构成,碳酸钙微晶体排列有一定的取向,较为复杂。海口和陵水两地藤壶壳板中碳酸钙含量没有显著差异(P<0.05),最大值为93.87%,最小值90.77%。尽管三种碳酸钙含量测量法的结果没有显著性差异(P<0.05),但进一步综合分析发现钙黄绿素法测定结果最为稳定,且简单易操作,可作为首选方法。The microstructure of wall plate of the barnacle （Balanus reticulates Utinomi） was studied, and the cal- cium carbonate content of barnacle wall plate samples from two sampling sites was compared by three testing methods including calcein method, calcium carboxylic acid indicator method and mordant black T method. It was found that the microstructure of the wall plate is mainly composed of calcite, and the array of crystalline is most probably arranged in a specific complicated form. The maximum value of the calcium carbonate content is 93.87% and the minimum is 90.77%. There is no significant difference （P〈0.05） between two sampling sites, and also no signifi- cant difference （P〈0.05） among the three testing methods. However, from the point of the view of testing stability and operability, calcein method is the best technique for the examination of calcium carbonate content of barnacle shell.

关 键 词：网纹藤壶(Balanus reticulates Utinomi) 壳板 矿化 碳酸钙 

分 类 号：Q959.223[生物学—动物学]