DNA修复基因多态性与二甲基甲酰胺致肝功能损伤关系的研究  

Relationship Between Polymorphisms of Gene XRCC1 and Dimethylformamide-induced Liver Toxicity

作　　者：吴京颖[1] 许旭艳[1] 刘祥铨[1] 洪惠民[1] 何颖荣[1] 张伟[1] 刘合焜[2] 

机构地区：[1]福州市疾病预防控制中心,福建福州350004 [2]福建医科大学基础医学院,福建福州350004

出　　处：《预防医学论坛》2013年第7期485-488,共4页Preventive Medicine Tribune

基　　金：福建省卫生厅青年科研课题(2010-2-82)

摘　　要：目的研究DNA修复基因(XRCC1)的单核苷酸多态性与二甲基甲酰胺肝毒性易感性的关系。方法 2010年9月至2011年7月,随机抽取福州市3家人造革生产企业,对PU革生产车间进行作业场所空气中二甲基甲酰胺浓度检测;对全部二甲基甲酰胺作业工人进行问卷调查和血丙氨酸氨基转氨酶(ALT)、天门冬氨酸氨基转氨酶(AST)、谷氨酰氨基转肽酶(GT)水平和XRCC1基因基因型检测。结果检测3家企业的9类二甲基甲酰胺作业工种的110个作业岗位,空气中二甲基甲酰胺浓度为(59.6±21.7)mg/m3,有72个作业岗位超出职业接触限值(TWA 20mg/m3),超标率为65.45%。检测296名作业工人,肝功能异常(ALT≥50U/L、AST≥40U/L、GT≥54U/L中1项或1项以上)的78人,异常率为26.35%;XRCC1基因分为XRCC1-194CC组与CT+TT组、XRCC1-280GG组与GA+AA组,XRCC1-399GG组与GA+AA组,各基因型的分布频率符合遗传学的Hardy-Weinberg平衡(P>0.05);不同基因型组(XRCC1-194的CC与CT+TT、XRCC1-280的GG与GA+AA、XRCC1-399的GG与GA+AA)工人血ALT、AST、GT水平的差异均无统计学意义(P>0.05),肝功能异常率的差异均无统计学意义(P>0.05)。结论 XRCC1基因Arg194Trp、Arg280His、Arg399Gln多态性可能与二甲基甲酰胺肝毒性易感性无关。Objective To explore the relationship between gene polymorphisms of XRCCl and susceptibility to dime thylformamide-induced liver toxicity. Methods Dimethylformamide consistency was detected from PU leather work- place in 3 tanneries,Fuzhou city, during September, 2010-July, 2011. All dimethylformamide-exposed workers were taken for questionnaire survey,and ALT, AST,GT levels in blood and XRCC1 genotype were analyzed. Results Dimethyl- formamide consistency was 59.6±21.7 mg/m^2 in 110 posts of 9 types of dimethylformamide work categories in 3 tanner ies. Dimethylformamide consistency in 72 posts exceeded the occupational exposure limit （TWA 20 mg/ma） ,and the over proofrate was 65.45%. There were 78 workers suffering from high liver function lever （ALT≥50 IU/L or ASTT≥40 IU/ L orGT≥54 IU/L） in 296 dimethylformamide-exposed workers, the incidence rate was 26.35~. The distribution fre quencies of alleles and genotypes of XRCC1 in 296 dimethylformamide-exposed workers accorded with Hardy-Weinberg e- quilibrium （ P 〉0.05）, the differences of blood ALT, AST, GT levels among different genetypes （XRCC1 194CC/CT ＋ TT,XRCC1-280GG/GA q- AA, XRCC1-399GG/GA ＋ AA） showed no statistical significance （ P 〉 0.05 ）, and the inci- dence rate of high liver function lever between the groups above-mentioned respectively had no statistical significance. Conclusion The XRCC1Arg194Trp, Arg280His, Arg399Gln polymorphisms are probably not the susceptible factors to dimethylformamide-induced liver toxicity.

关 键 词：XRCC1基因 多态性 二甲基甲酰胺 肝毒性 易感性 

分 类 号：R134.4[医药卫生—劳动卫生]