水稻白叶枯病抗性基因Xa31(t)候选基因表达载体的构建  

Construction of Expression Vector for Candidate Gene of Bacterial Blight Resistance Gene Xa31(t) in Rice

作　　者：赵传纪 刘学群[1] 王春台[1] 徐鑫[1] 

机构地区：[1]中南民族大学生命科学学院/生物技术国家民委重点实验室,武陵山区特色资源植物种质保护与利用湖北省重点实验室,湖北武汉430074

出　　处：《安徽农业科学》2013年第15期6611-6612,6629,共3页Journal of Anhui Agricultural Sciences

基　　金：教育部科学技术研究重点项目(210265)

摘　　要：[目的]构建水稻白叶枯病抗性基因Xa31(t)候选基因的表达载体。[方法]利用长片段PCR在抗白叶枯病水稻品种扎昌龙中扩增长10.6 kb的候选基因,将其通过Asc I单酶切克隆至植物表达载体pCAMBIA1300中,并对阳性克隆进行质粒PCR、酶切检测和测序分析。[结果]试验成功构建了水稻白叶枯病抗性基因Xa31(t)候选基因的表达载体。[结论]该研究为进一步研究Xa31(t)基因的功能奠定了基础。[Objective] The paper was to construct the expression vector for candidate gene of the bacterial blight resistance gene Xa31（t） in rice.[Method] A 10.6 kb-length candidate gene was amplified form the genomic DNA of bacterial blight resistance rice cultivar ’Za Chang Long’（ZCL） by long-range PCR,and the gene was cloned into plant expression vector pCAMBIA1300 by Asc I digestion.The positive clones were detected by plasmid PCR,restriction enzyme digestion and sequencing.[Result] The expression vector for candidate gene of the bacterial blight resistance gene Xa31（t） in rice was constructed successfully.[Conclusion] The research laid a foundation for the further study of the function of Xa31（t） gene.

关 键 词：水稻 白叶枯病 候选基因 长片段PCR 表达载体 

分 类 号：S511[农业科学—作物学]