绿色木霉外源基因表达系统的构建  

Construction of Heterologous Gene Expression System of Trichoderma viride

作　　者：刘芳[1] 杨佳颖[1] 陈红漫[1] 

机构地区：[1]沈阳农业大学生物科学技术学院,沈阳110866

出　　处：《湖北农业科学》2013年第13期3180-3183,共4页Hubei Agricultural Sciences

摘　　要：利用PCR方法克隆瑞氏木霉(Trichoderma reesei)外切葡聚糖纤维二糖水解酶Ⅰ基因(cbhⅠ)启动子序列,以pSK载体为骨架,构建了pSK-cbhⅠ-GFP-hph表达载体,并成功转入到绿色木霉(T.viride)Sn-9106中。通过对绿色木霉Sn-9106进行遗传改造,为纤维素酶基因在木霉中同源重组表达提供遗传转化平台。Promoter of cellobiohydrolase I gene （cbh I ） of Trichoderma reesei was cloned by PCR. The expression vector pSK-cbh I-GFP-hph was constructed with pSK vector as backbone. Then it was introduced into the strain of T. viride Sn-9106 by transformation. The genetic modification of T. viride Sn-9106 could provide genetic transformation platform for homologous recombination expression of cellulase gene in T. spp..

关 键 词：绿色木霉(Trichoderma viride) cbhⅠ启动子 绿色荧光蛋白 外源基因表达 

分 类 号：Q786[生物学—分子生物学] Q939.5