置换型荧光偏振免疫检测除草剂丁草胺  

Displacement Fluorescence Polarization Immunoassay for Herbicide Butachlor

作　　者：雷红涛[1] 吴青[1] 卢蓝蓝[1] 刘英菊[2] 薛钢[1] 王强 徐振林[1] 孙远明[1] 

机构地区：[1]华南农业大学食品学院广东省食品质量安全重点实验室,广州510642 [2]华南农业大学食品学院理学院生物材料研究所,广州510642

出　　处：《分析化学》2013年第7期1031-1036,共6页Chinese Journal of Analytical Chemistry

基　　金：行业(农业)公益项目(No.201003008-08);国家自然科学基金项目(Nos.30700663;21105030);高等学校博士点基金项目(No.20114404130002);广州市科技计划项目(No.11C12100483)资助

摘　　要：建立了一种置换型荧光偏振免疫分析方法(DFPIA)检测水样中除草剂丁草胺。研究了不同结构示踪物对丁草胺DFPIA灵敏度的影响,优化筛选出一种荧光示踪物抗体组成的免疫复合物;考察了DFPIA置换反应动力学。所建立的DFPIA法特异性良好,检出限为22.6μg/L,半数抑制浓度(IC50)为321.3μg/L,检测范围为60～1472 mg/L,检测时间为12.5 min,添加回收率在88.1%～112.5%间,免疫复合物可以在4℃下稳定保存至少1星期以上。本方法适于环境水样中丁草胺的筛选检测。A displacement fluorescence polarization immunoassay （DFPIA） was developed for the detection of herbicide butachlor. The influence of the tracer structure on the assay performance was examined and an immunocomplex of tracer with longest arm was screened. The displacement reaction curves showed a kinetic nature of second-order reaction. The limit of detection （LOD） was 22.6 μg/L with 321.3 μg/L of IC50 and 59.6-1472.1 μg/L of working range. The detection time was only 12.5 min, and the recovery ranged from 88.1% to 112.5%. The immunocomplex was stable for more than one week at 4 ℃. The proposed DFPIA was suitable as a surveillance screening method for the detection of the herbicide butachlor in environmental water samples.

关 键 词：荧光偏振免疫分析 丁草胺 

分 类 号：X832[环境科学与工程—环境工程] O657.3[理学—分析化学]