影响禽致病性大肠杆菌信号分子AI-2产生的因素分析  

Analysis of Factors Affecting the Activity of AI-2 of Avian Pathogenic Escherichia coli

作　　者：白灏[1,2] 韩先干[1] 刘蕾[1] 祁克宗[2] 刘海文[1] 丁铲[1] 于圣青[1] 

机构地区：[1]中国农业科学院上海兽医研究所,上海200241 [2]安徽农业大学动物科技学院,合肥230036

出　　处：《中国农业科学》2013年第15期3220-3226,共7页Scientia Agricultura Sinica

基　　金：国家自然科学基金(31001078,31072161,30871851)

摘　　要：【目的】探讨禽致病性大肠杆菌(Avian Pathogenic Escherichia coli,APEC)在不同生长时期和不同培养条件下,信号分子AI-2(Autoinducer-2,AI-2)的合成与luxS和pfs的转录水平之间的关系。【方法】利用哈维弧菌BB170(vibrio harveyi BB170)检测不同生长时期和不同培养条件下APEC的AI-2活性。同时利用Real-time PCR检测APEC不同生长时期和不同培养条件下luxS和pfs的转录水平。【结果】APEC在不同生长时期的AI-2活性与luxS的转录水平保持一致。培养基中加入葡萄糖,麦芽糖和NaCl后,AI-2活性和luxS转录水平都上调,加入蔗糖后则都下调,两者保持一致性。AI-2活性与pfs的转录水平则并不一致。【结论】APEC的AI-2产生水平与luxS的转录水平有高度的相关性,而与pfs的转录水平没有显著相关性;葡萄糖、麦芽糖和NaCl,促进AI-2的合成。【Objective】 In this study,the relationship between the transcription level of luxS and pfs and AI-2 production in different growth phases and culture conditions were analyzed.【Method】 AI-2 activity in different growth phases and culture conditions was measured using the V.harveyi bioluminescence assay.The levels of luxS and pfs mRNA were analyzed using real-time PCR.【Result】 The results showed that the level of AI-2 was consistent with the level of luxS mRNA in different growth phases.AI-2 production and luxS mRNA by APEC was increased by supplementation of glucose,maltose,and NaCl,while the addition of sucrose was decreased.The transcription level of luxS was correlated to the level of AI-2 production,while the transcription level of pfs did not correlate with the level of AI-2 production.【Conclusion】 The level of transcription of luxS is highly correlated with the level of AI-2 production,while the level of transcription of pfs does not correlate with the level of AI-2 production.Glucose,maltose and NaCl accelerate the synthesis of AI-2.

关 键 词：禽致病性大肠杆菌 信号分子AI-2 LUXS PFS REAL-TIME PCR 

分 类 号：S852.61[农业科学—基础兽医学]