糖基化终产物对心肌细胞GLP-1受体表达及凋亡影响的研究  

Effects of advanced glycation end-products on the expression of GLP-1 receptor and apopotis in cultured cardiomyocytes

作　　者：胡波[1,2] 李德才[1,2] 

机构地区：[1]川北医学院附属第二医院 [2]绵阳404医院心内科

出　　处：《四川医学》2013年第6期746-748,共3页Sichuan Medical Journal

摘　　要：目的探讨糖基化终产物(AGEs)对乳鼠心肌细胞胰高血糖素样肽1受体(GLP-1R)、caspase-3表达及凋亡的影响。方法原代培养乳鼠心肌细胞,以不同浓度葡萄糖孵育的糖化清蛋白(AGE-BSA)干预24h和同一浓度葡萄糖孵育的AGE-BSA干预24～72h,检测其对心肌细胞GLP-1RmRNA、活化的caspase-3表达及凋亡的影响。结果 AGE-BSA可抑制心肌细胞GLP-1RmRNA表达,并诱导细胞caspase-3表达,凋亡增加。各AGE-BSA组间及与对照组比较差异有统计学意义(P<0.05)。结论 AGE-BSA能抑制心肌细胞GLP-1RmRNA表达,并诱导细胞caspase-3表达,增加细胞凋亡,提示糖基化终产物在糖尿病心肌病的发生中可能具有重要的作用。Objective To investigate the effects of AGEs on the expression of GLP-1 receptor and Caspase-3 protein and apopotis in cultured cardiomyocytes.Methods Primary cardiomyocytes were isolated from SD neonatal rats ventricles,myocytes were exposed to AGE-BSA for 24 to 72 hours.Determined the expressions of GLP-1 reccrptor mRNA and caspase-3 protein and the percentage of apoptosis.Results AGE-BSA decreased the expression of GLP-1 reccrptor mRNA（P0.05）.The expressions of Caspase-3 protein were increased（P0.05） and the percentage of apoptosis was apparent in AGE-BSA group（P0.05）.Conclusion AGE-BSA decreased the expression of GLP-1 reccrptor mRNA.AGE-BSA could increased the expression of Caspase-3 protein and apoptosis in cultured rat cardiomyocytes.These data suggesting that AGE-BSA play an important role in diabetic cardiomyopathy.

关 键 词：心肌细胞 糖基化终产物 胰高血糖素样肽1受体 凋亡 

分 类 号：R542.2[医药卫生—心血管疾病] R587.2[医药卫生—内科学]