超声-UPLC法测定补骨脂中补骨脂素和异补骨脂素  

Determination of Psoralen and Isopsoralen in Psoralea corylifolia by Ultrasonic-UPLC

作　　者：李凯[1] 牛乐[1] 李赫宇 贾利利[1] 张振凌[1] 

机构地区：[1]河南中医学院药学院,河南郑州450008 [2]天津市益倍建生物技术有限公司,天津300457

出　　处：《食品研究与开发》2013年第13期84-86,共3页Food Research and Development

基　　金：河南中医学院博士基金项目(BSJJ2010-06)

摘　　要：补骨脂是我国用于生产各种保健品的传统原料之一,其主要活性成分为补骨脂素和异补骨脂素。本实验改进中国药典补骨脂项下补骨脂素和异补骨脂素含量测定方法。采用C18ODS色谱柱(1.8μm,4.6 mm×50 mm),流动相乙腈-水(35∶65),流速0.5 mL/min,检测波长246 nm,测定药典法和超声法制备补骨脂供试品溶液中补骨脂素和异补骨脂素含量。5 min内补骨脂素、异补骨脂素分离良好。在相应浓度范围内2种化合物的线性关系良好,r>0.999 7;精密度RSD≤2%;平均加样回收率为>99%。超声法与索氏提取法相比无显著性差异(P>0.05)。该方法操作简便,节省时间和溶剂,为补骨脂中补骨脂素、异补骨脂素的分析提供了一种简便快速的新手段。To improve the Chinese pharmacopoeia method for determination of Psoralea corylifolia. C18ODS column （1.8 um, 4.6 mm x 50 mm） was adopt, the mobile phase was composed of acetonitrile and water （35：65） at flow rate of 0.5 mL/min, detective wavelength was 246 nm. Psoralen and isopsoralen in test solution prepared by pharmacopoeia and ultrasonic method was determined. Psoralen and isopsoralen was well separated in 5 min. The two kinds of compounds was well linear correlated within corresponding concentration range, r 〉 0.999 7 ; RSD≤2 % ; average recovery 〉99 %. There is no significant difference between ultrasonic method and Soxhlet extraction. The method significantly saved analysis time and solvent, and provided a simple, fast means for determination of psoralen and isopsoralen.

关 键 词：补骨脂 补骨脂素 异补骨脂素 超声法 

分 类 号：R286.0[医药卫生—中药学]