锶对大麻哈鱼稚鱼生长发育及肌肉ATP酶活性的影响  

Effects of Strontium Ion on Growth and ATPase Activity in Muscle of Chum Salmon(Oncorhynchus keta)Juveniles

作　　者：宋洪建[1,2] 刘伟[1] 王继隆[1] 唐富江[1] 

机构地区：[1]中国水产科学研究院黑龙江水产研究所,黑龙江哈尔滨150070 [2]东北农业大学动物科学技术学院,黑龙江哈尔滨150030

出　　处：《水产学杂志》2013年第4期23-28,共6页Chinese Journal of Fisheries

基　　金：农业部财政专项物种资源保护项目(20112130135);中央级公益性科研院所基本科研业务费专项(201107)资助;哈尔滨市科技局优秀学科带头人项目(2010RFXYNO47)

摘　　要：在实验室条件下,采用长期暴露的方法,研究锶(Sr2+)对大麻哈鱼(Oncorhynchus keta)稚鱼生长、存活率和肌肉Ca2+-ATP酶及Na+/K+-ATP酶活性的影响。在水温(15±0.2)℃下,将体质量(0.389±0.021)g的大麻哈鱼饲养在可控温玻璃水槽(100cm×50cm×40cm)中。水槽中添加SrCl2·6H2O,使Sr2+浓度达10、20、30和40mg·L-1,以不添加锶的水槽为对照组。30d饲养表明,各实验组大麻哈鱼稚鱼均能生长,但高质量浓度的锶不同程度地抑制了大麻哈鱼稚鱼的生长和存活率,显著影响了稚鱼肌肉中Ca2+-ATP酶和Na+/K+-ATP酶的活性。锶(Sr2+)质量浓度为10 mg·L-1时,各项生长指标和存活率最高;随着锶(Sr2+)质量浓度的升高,Ca2+-ATP酶和Na+/K+-ATP酶的活性下降。结果提示,大麻哈鱼稚鱼长期暴露的锶安全质量浓度在10mg·L-1之内。The effects of Sr2＋ on growth, survival and Ca2＋-ATPase, and Na＋/K＋-ATPase activities in muscle were studied in chum salmon （Oncorhynchus keta） juveniles in a laboratory. The chum salmon juveniles with body weight of （0.389±0.021 ） g were reared in a tank in which SrCl2·6H2O was added at a Sr2＋mass concentration of 0 （as a control）, 10 mg·L^-1, 20 mg·L^-1, 30 mg·L^-1 and 40 mg· L^-1 for 30 days with triplication at water temperature of （ 15 ± 0.2）℃. Results showed that the chum salmon juveniles in each experi- mental group grew within the range of exposure dosages, the maximal growth and survival in the case of Sr2＋ mass concentration of 10 mg·L^-1. The muscular Ca2＋-ATPase and Na＋/K＋-ATPase activities were found to be significantly inhibited by Sr2＋ ion, with the in- creased in the mass concentration of Sr2＋, Ca2＋-ATPase and Na＋/K＋-ATPase activities showing a downward trend. It is concluded that safe concentration of Sr2＋ ion for long term exposure is within 10 mg·L^-1 for chum salmon juveniles exposed to Sr2＋ ion.

关 键 词：Sr2+ 大麻哈鱼稚鱼 生长指标 CA2+-ATP酶 Na+ K+-ATP酶 

分 类 号：S965.229[农业科学—水产养殖]