Biodegradation of ferulic acid by a newly isolated strain of Cupriavidus sp. B-8  

作　　者：CHAI Li-yuan ZHANG Huan YANG Wei-chun ZHU Yong-hua YANG Zhi-hui ZHENG Yu CHEN Yue-hui 

机构地区：[1]National Engineering Research Center for Pollution Control of Heavy Metal,(Institute of Environmental Science and Engineering, School of Metallurgical Science and Engineering, Central South University), Changsha 410083, China [2]State Key Laboratory of Chemo/Biosensing and Chemometrics College of Biology, Hunan University), Changsha 410082, China

出　　处：《Journal of Central South University》2013年第7期1964-1970,共7页中南大学学报（英文版）

基　　金：Foundation item： Project（50925417） supported by the National Science Fund for Distinguished Young Scholars of China; Project（50830301） supported by the National Natural Science Foundation of China; Project（2011467062） supported by National Research Fund for Public Benefit （Environmental Protection） Industries, China

摘　　要：As a major component of lignin and abundantly existing in softwood and hardwood, ferulic acid has been used as a lignin-related compound for lignin biodegradation study. Biodegradation of ferulic acid by Cupriavidus sp. B-8, a newly isolated strain, was studied. This strain is able to utilize a wide range of lignin-related aromatic compounds as the sole carbon and energy source, including guaiacol, veratric acid, vanillic acid, cinnamic acid, p-coumaric acid, ferulic acid, and sinapic acid. In addition, the effects of different concentrations of ferulic acid on growth of Cupriavidus sp. B-8 were studied. The growth of Cupriavidus sp. B-8 is better under the condition of lower concentration. High-performance liquid chromatography （HPLC） analysis reveals that above 95% of ferulic acid is degraded within 12 h by Cupriavidus sp. B-8. Based on identification of biodegradation intermediates and further metabolites, the biodegradation pathway of ferulic acid by Cupriavidus sp. B-8 was proposed. Ferulic acid is initially converted to 4-vinylguaiacol, and further oxidized to vanillic acid and protocatechuic acid.在软木材和硬木作为木质素并且富有地的一个主要部件存在， ferulic 酸为木质素 biodegradation 学习被用作木质素相关的混合物。由 Cupriavidus sp 的 ferulic 酸的 Biodegradation。B-8，最新孤立的紧张，被学习。这紧张能作为唯一的碳和精力来源利用大量木质素相关的芳香的混合物，包括 guaiacol， veratric 酸，香子兰的酸的、苯乙烯的酸， p-coumaric 酸， ferulic 酸，和 sinapic 酸。另外， Cupriavidus sp 的生长上的 ferulic 酸的不同集中的效果。B-8 被学习。Cupriavidus sp 的生长。B-8 在更低的集中的条件下面更好。高效的液体层析(HPLC ) 分析表明在 95% ferulic 上面，酸被 Cupriavidus sp 在 12 h 以内降级。B-8。基于 biodegradation 中介和进一步的代谢物的鉴定，由 Cupriavidus sp 的 ferulic 酸的 biodegradation 小径。B-8 被建议。Ferulic 酸开始被变换成 4-vinylguaiacol，并且进一步氧化了到香子兰的酸和 protocatechuic 酸。

关 键 词：Cupriavidus sp. B-8 lignin biodegradation ferulic acid gas chromatography and mass spectrometry 

分 类 号：TQ245.24[化学工程—有机化工] X172