术前筛查HBeAg阴性HBV感染者检测乙肝大蛋白的意义  

Clinical significance of detecting serum hepatitis B virus large surface protein in preoperative screening in patients with HBeAg-negative hepatitis B infection

作　　者：史兵伟 田晓静 虞丽娟 

机构地区：[1]常州市中医院检验科,江苏常州213003

出　　处：《海南医学》2013年第16期2412-2414,共3页Hainan Medical Journal

摘　　要：目的了解肝功能正常、HBeAg阴性HBV感染的术前筛查患者乙肝大蛋白(HBV-LP)阳性情况,评判血清HBV-LP检测在这类乙型肝炎患者中的意义。方法使用ELISA法检测180例HBeAg阴性HBV感染的术前筛查患者HBV-LP、乙肝前S1(HBVpreS1),同时采用实时定量PCR法做HBVDNA水平检测。结果 180份HBeAg阴性HBV感染血清PreS1-Ag、HBV-LP和HBVDNA检测阳性率分别为21.1%、34.4%和32.7%,χ2检验显示三组检测阳性率差异有统计学意义(χ2=9.1457,P=0.0103),用Scheffe法做率的两两比较显示:PreS1-Ag检测阳性率明显低于HBV-LP和HBVDNA(P<0.05),而HBV-LP和HBVDNA检测阳性率差异无统计学意义(P>0.05)。HBV-LP和HBVDNA配对检测显示结果差异无统计学意义(χ2=0.1739,P=0.6767),同时相关性分析示良好相关性(χ2=95.655,P=0.0000,r=0.7217)。49例HBVDNAcopies/ml常用对数值与HBV-LP定量OD值的散点图显示正相关(r=0.6192)。结论对术前筛查HBeAg阴性乙型肝炎携带者,HBV-LP在反应病毒复制方面是一个优于HBVpreS1检测的良好指标,与HBVDNA检测有良好的相关性,在不能开展HBVDNA检测的基层单位可作为评价乙型肝炎传染性的一种替代与补充。Objective To detect serum hepatitis B virus large surface protein （HBV-LP） in preoperative screening in subjects with normal liver function and patients with HBeAg-negative hepatitis B infection,and to evaluate the significance of serum HBV-LP in the patients with hepatitis B.Methods HBV-LP,hepatitis B S1 （HBVpreS1） were measured by ELISA,and HBV DNA levels were detected by fluorescence quantitative PCR in 180 HBeAg-negative HBV patients in preoperative screening.Results In the 180 patients,the positive rates of PreS1-Ag,HBV-LP and HBV DNA were 21.1％,34.4％ and 32.7％,respectively.x2 test showed that the positive rates of three groups had significant difference （x2=9.145 7,P=0.010 3）.Scheffe test showed that the positive rate of PreS1-Ag detection was significantly lower than that of HBV-LP and HBV DNA （P＜0.05）,while no significant difference was found in the positive rates of HBV-LP and HBV DNA detection （P＞0.05）.HBV-LP and HBV DNA showed no significant differences in detection results （x2=0.173 9,P=0.676 7）.At the same time,correlation analysis showed a good correlation between HBV-LP and HBV DNA detection （x 2=95.655,P=0.0000,r=0.721 7）.The scatter plot of 49 cases ofHBV DNA copy number per ml （copies/ml） commonly used numerical and quantitative HBV-LP OD showed a positive correlation （r=0.6192）.Conclusion HBV-LP,superior to HBVpreS1,is a good indicator in response to viral replication.There was a good correlation between HBV-LP and HBV-DNA detection,which can be used as a substitute and supplement of hepatitis B infection in hospital.

关 键 词：乙型肝炎病毒 乙肝大蛋白(HBV-LP) 乙肝DNA(HBV-DNA) 乙肝前S1抗原(PreS1-Ag) 

分 类 号：R512.62[医药卫生—内科学]