枯草芽孢杆菌CS16诱导香蕉抗病性相关防御酶系的研究  

Induction of Defense-related Enzymes in Banana Seedling by Bacillus subtilis Strain CS16

作　　者：林陈强[1] 李占飞[1,2] 张慧[1] 林戎斌[1] 林新坚[1] 陈济琛[1] 

机构地区：[1]福建省农业科学院土壤肥料研究所,福建福州350003 [2]福建农林大学生命科学学院,福建福州350002

出　　处：《福建农业学报》2013年第6期570-574,共5页Fujian Journal of Agricultural Sciences

基　　金：福建省自然科学基金项目(2009J01096);福建省科技计划项目--省属公益类科研院所基本科研专项(2009R10032-2);福建省种业创新与产业化工程项目(2011FJZY-9-07);农业部公益性行业科研专项(201303094)

摘　　要：以多酚氧化酶(PPO)、过氧化物酶(POD)、超氧化物歧化酶(SOD)、苯丙氨酸解氨酶(PAL)、β-1,3-葡聚糖酶等5种防御酶作为植物抗病性反应指标,研究枯草芽孢杆菌CS16发酵液及其上清液对香蕉体内防御酶活性的影响,探讨其诱导香蕉抗病性机理。结果表明:CS16发酵液和上清液处理香蕉苗后,均能诱导香蕉叶片中PPO、POD、SOD、PAL、β-1,3-葡聚糖酶等5种防御酶活性变化。CS16发酵液处理可诱导香蕉植株防御酶出峰数目多、持续时间长。说明CS16菌株及其胞外代谢物在一定程度上能够诱导香蕉抗病性,推测CS16菌株诱导香蕉抗病性是其生防机制之一。To probe into the biocontrol mechanism of Bacillus subtilis strain CS16and develop effective biocontrol agents,the activity of polyphenol oxidase（PPO）,peroxidase（POD）,superoxide dismutase（SOD）,phenylalanine（PAL）andβ-1,3-glucanase of banana were characterized after being inoculated with fermented liquid of CS16and its cell-free culture supernatant.The results showed that inoculated with fermented liquid of CS16and its cell-free culture supernatant will increase the activities of PPO,POD,SOD,PAL andβ-1,3-glucanase in banana leaves,and the increase activities induced by fermented liquid of CS16is better.Our results suggested that Bacillus subtilis strain CS16and its extracellular metabolites could induce disease resistance of banana seedling,and the induced systemic resistance is one of the biocontrol mechanisms of the strain CS16against Banana vasicular wilt.

关 键 词：枯草芽孢杆菌 香蕉枯萎病 诱导系统抗性 生防机制 

分 类 号：S432[农业科学—植物病理学] S476[农业科学—农业昆虫与害虫防治]