车前子提取物抑制大鼠前列腺增生及对5α-还原酶的影响  

Extract of plantain seed inhibiting benign prostatic hyperplasia of rat and its effect on 5-alpha reductase

作　　者：王毓平[1] 李小林[1] 刘永青[1] 杨家大[1] 胡有生[1] 梁生林[1] 

机构地区：[1]井冈山大学医学院,江西吉安343009

出　　处：《中国老年学杂志》2013年第15期3643-3646,共4页Chinese Journal of Gerontology

基　　金：江西省教育厅科学技术研究资助项目(No.GLL10546)

摘　　要：目的探讨车前子提取物抗大鼠前列腺增生的效应及其可能机制。方法用去势雄性SD大鼠皮下注射丙酸睾酮造模,按体重随机分组,用不同浓度的车前子提取物进行灌胃;28 d后处死大鼠,取前列腺组织;用生理盐水替代法测量前列腺体积,电子天平称取前列腺湿重、并分别计算前列腺指数;用HE染色显微观察前列腺组织病理学改变;用酶联免疫法(ELISA)测定前列腺组织双氢睾酮含量。结果车前子提取物显著降低良性前列腺增生模型的前列腺湿重和前列腺指数(P<0.05或P<0.01),减轻了前列腺组织的增生性改变;车前子提取物还降低了前列腺组织双氢睾酮含量(P<0.05或P<0.01)。结论车前子提取物具有抑制良性前列腺增生的作用,其抑制作用与抑制前列腺组织中的5α-还原酶活性,降低组织中双氢睾酮的含量有关。Objective To study the inhibiting effects of extract of plantain seeds on prostatic hyperplasia （PH） in rat with benign PH （BPH） and explore its possible mechanisms. Methods Rat models with BPH were made by subcutaneously injecting testosterone propi- onate for 28 d after being castrated and randomly divided 4 groups by weight ： model, finastefid, low and high dose groups of extract of plantain seeds, the other group was normal control. Different doses of extract of plantain seeds were administered respectively by garage, the rats were put to death and prostatic glands were took after 28 d. Prostatic size was measured with normal saline substitution method. Prostatic weight was taken by electronic balance and prostate index was calculated. Pathology change of prostate tissue was observed with HE staining. The dihydrotestosterone content from the prostate gland was determined by ELISA. Results Extract of plantain seeds significantly reduced prostatic weight and index in rat with BPH （ P 〈 0. 05 or P 〈 0. 01 ） , and alleviated the proliferative changes in prostate. Extract of plantain seeds reduced dihydrotestosterone contents in prostate tissues （ P 〈 0. 05 or P 〈 0.01 ）. Conclusions Extract of plantain seeds has inhibitory effect on benign prostatic hyperplasia, and the inhibition may relate to inhibiting the 5 alpha-reductase activity and reducing the di- hydrotestosterone content in prostate.

关 键 词：车前子 良性前列腺增生 5Α-还原酶 双氢睾酮 

分 类 号：R285.5[医药卫生—中药学]