人参皂苷Rg1促进人牙周膜干细胞增殖与成骨分化  

Promotion of ginsenoside Rg1 on proliferation and osteogenic potential of human periodontal ligament stem cells

作　　者：王萍[1] 周玥[1] 王亚平[1] 屈晨[1] 

机构地区：[1]重庆医科大学附属第一医院口腔科,重庆400016

出　　处：《第三军医大学学报》2013年第15期1566-1569,共4页Journal of Third Military Medical University

基　　金：重庆市科委自然科学基金(CSTC2011jjA10007);重庆市卫生局科研项目(2011-2-056)~~

摘　　要：目的观察人参皂苷Rg1对体外培养的人牙周膜干细胞(periodontal ligament stem cells,PDLSCs)增殖和成骨分化的影响。方法选取第3代PDLSCs,采用MTT法和细胞周期检测不同浓度(0.25、0.5、1、2、4μmol/L)人参皂苷Rg1对PDLSCs增殖的影响;采用碱性磷酸酶(ALP)活性、实时荧光定量RT-PCR和放免法检测人参皂苷Rg1对PDLSCs成骨分化的影响;采用ELISA方法检测人参皂苷Rg1对PDLSCs碱性成纤维细胞生长因子(basic fibroblast growth factor,bFGF)表达的影响。结果与对照组比较,浓度为0.5、1、2μmol/L的人参皂苷Rg1能明显提高PDLSCs的增殖能力(P<0.05),其中浓度为1μmol/L的促增殖作用最强,S+G2/M期细胞百分率明显高于对照组(P<0.05);浓度为1μmol/L的人参皂苷Rg1组ALP活性、骨钙素(osteocalcin,OCN)和bFGF的表达水平明显高于对照组(P<0.05)。结论人参皂苷Rg1对体外培养的PDLSCs有促进增殖和成骨分化的作用。Objective To evaluate the effect of ginsenoside Rg1 on the proliferation and osteogenic potential of human periodontal ligament stem cells（PDLSCs）.Methods PDLSCs at passage 3 were incubated with different concentrations of ginsenoside Rg1（0.25,0.5,1,2 and 4 μmol / L）.The effect of ginsenoside Rg1 on the proliferative ability of PDLSCs was evaluated by MTT assay,and flow cytometry was used for detecting cell cycle.The osteogenic potential of the cells was assayed by ALP enzymatic activity,realtime RT-PCR and radioimmunoassay,respectively.Basic fibroblast growth factor（bFGF） was detected by enzyme-linked immunosorbent assay.Results Compared with the control group,the proliferative ability of PDLSCs in ginsenoside Rg1 groups（0.5,1 and 2 μmol / L） was significantly enhanced（P 0.05）,especially in the ginsenoside Rg1 group（1 μmol / L）.Cell cycle analysis showed that ginsenoside Rg1（1 μmol / L） significantly increased the proportion of PDLSCs in proliferative phase（S ＋ G2/ M phase）.ALP activity and the expression of osteocalcin（OCN） and bFGF significantly were increased in ginsenoside Rg1 group（1 μmol / L） as compared with the control group（P 0.05）.Conclusion Ginsenoside Rg1 can promote the proliferation and osteogenic potential of PDLSCs in vitro.

关 键 词：人参皂苷RG1 牙周膜干细胞 增殖 成骨 

分 类 号：R285.5[医药卫生—中药学] R322.41[医药卫生—中医学]