醛糖还原酶5’端(AC)n二核苷酸重复序列多态性与2型糖尿病合并冠心病的相关性  

Relationship between an (AC)-n dinucleotide repeat polymorphic marker at 5'end of the aldose reductase gene and type 2 diabetes mellitus with coronary heart disease

作　　者：薛亚梅[1] 刘长山[2] 王秀军[2] 雷佩佩[1] 

机构地区：[1]潍坊医学院研究生部,山东潍坊261053 [2]山东省潍坊市人民医院内分泌科,山东潍坊261041

出　　处：《中国医药科学》2013年第15期21-22,82,共3页China Medicine And Pharmacy

基　　金：山东省医药卫生科技发展计划(保健项目)(548945)

摘　　要：目的本研究旨在调查山东地区汉族人群醛糖还原酶（AR）5’端（AC） n 二核苷酸重复序列多态性,探讨AR5’端（AC ）n重复序列多态性与2型糖尿病（DM ）、2型糖尿病合并冠心病（CHD ）之间的关系。方法随机选取新诊断2型糖尿病患者74例,2型糖尿病合并冠心病者68例,正常对照组116例。应用聚合酶链式反应（PCR ）、琼脂糖凝胶电泳技术、聚丙烯酰胺凝胶电泳技术及DNA测序检测AR等位基因，比较各组等位基因分布频率。结果3组均发现7种等位基因（Z-6 - Z＋6）,各组间等位基因分布频率差异无统计学意义。结论AR基因5’端（AC）n 二核苷酸重复序列多态性等位基因不是糖尿病、糖尿病合并冠心病发病的独立危险因素。Objective To investigate AR 5,end （AC）n polymorphism in Han Nationality population in Shandongdistrict,and discuss its relationship between type 2 diabetes（T2DM）,type 2 diabetes with coronary heart disease（CHD）.Methods 74 patients with newly diagnosed type 2 diabetes mellitus, type 2 diabetes mellitus with coronary heartdisease in 68 cases, 116 cases of normal control group were randomly selected. Polymerase chain reaction （PCR）,agarose gel electrophoresis, polyacrylamide gel electrophoresis and DNA sequencing were used to detect AR alleles,allele frequency distribution in each group were compared. Results Three groups were found seven alleles （Z-6-Z＋6）,there were no obvious difference of allele frequency distribution between groups. Conclusion AR 5＇end （AC）n polymorphismsmay not the independent risk factors for diabetes mellitus, and diabetes combined with coronary heart disease.

关 键 词：2型糖尿病 醛糖还原酶 基因 多态性 冠心病 

分 类 号：R587[医药卫生—内分泌]