左旋精氨酸对骨质疏松骨折骨愈合及血生化的影响  

L-arginine effects on osteoporotic fracture healing and blood biochemistry

作　　者：张世旭[1] 路考生[1] 石昌浩[1] 樊焱[1] 姚志伟[1] 尹芸生[1] 

机构地区：[1]山西医科大学第二附属医院骨科,山西省太原市030001

出　　处：《中国组织工程研究》2013年第28期5120-5125,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:一氧化氮在骨代谢中具有重要作用,不同剂量一氧化氮底物左旋精氨酸对骨质疏松骨折的愈合作用尚不明确。目的:观察不同剂量左旋精氨酸对大鼠骨质疏松性骨折骨愈合及血生化的影响。方法:6月龄雌性Wistar大鼠50只随机分为假手术组10只和骨质疏松模型组(双侧卵巢切除)40只,待骨质疏松模型组造模成功后,将50只大鼠全部制作左股骨干中段骨折模型,骨质疏松模型组再随机分为4组,低、中、高剂量左旋精氨酸组于术后第2天分别腹腔注射1,3,5mg/kg左旋精氨酸,骨质疏松对照组和假手术组注射同体积生理盐水。于给药8周后测量各组大鼠腰椎及骨痂骨密度,血清一氧化氮、一氧化氮合酶,取骨痂行组织学检查。结果与结论:骨质疏松大鼠在骨折愈合过程中,血清一氧化氮、一氧化氮合酶较正常骨折大鼠低(P<0.05)。左旋精氨酸能提高骨质疏松骨折大鼠血清一氧化氮及一氧化氮合酶浓度,并且中剂量的左旋精氨酸还能提高骨质疏松大鼠的骨痂骨密度和腰椎骨密度,使骨小梁粗壮,排列紧密有序。结果表明3mg/kg左旋精氨酸能促进骨质疏松骨折愈合,同时改善骨质疏松。METHODS： A total of 50 female Wistar rats, aged 6-month-old, were randomly divided into sham-surgery （n=10） and osteoporosis （bilateral ovariectomy, n=40）. After osteoporosis model was established, left middle femoral fractures were made in all 50 rats, and the osteoporosis group was further divided into four groups, low, middle and high dose of L-arginine, and control groups. The L-arginine groups were intraperitoneally injected with 1, 3, and 5 mg/kg L-arginine at the second day following surgery, while the control and sham-surgery groups were injected with the same volume of normal saline. At 8 weeks, the lumbar and callus bone mineral density, serum nitric oxide, and nitric oxide synthase were detected. Meanwhile, the bone callus histological examination was conducted. RESULTS AND CONCLUSION： During fracture healing, osteoporosis rats showed a low level of serum nitric oxide and nitric oxide synthase compared with normal fractured rats （P 〈 0.05）. L-arginine can improve the concentration of serum nitric oxide and nitric oxide synthase in osteoporosis rats. Moreover, middle dose of L-arginine can increase the callus and lumbar spine bone mineral density of osteoporosis rats, so the number and continuity of bone trabecula were enhanced. These findings suggest that middle dose of L-arginine （3 mg/kg） can promote healing process of osteoporotic fractures and improve osteoporosis.

关 键 词：组织构建 骨组织构建 左旋精氨酸 骨质疏松 骨折 骨愈合 一氧化氮 一氧化氮合酶 骨密度 骨痂 

分 类 号：R318[医药卫生—生物医学工程]