缺血再灌流股骨头骺影响关节软骨细胞的凋亡  

Does ischemia/reperfusion impact apoptosis of articular chondrocyte in the femoral head epiphyses

作　　者：张敬东[1] 易先宏[1] 李玉安[1] 

机构地区：[1]温州医学院附二院骨科,浙江省温州市325027

出　　处：《中国组织工程研究》2013年第28期5133-5138,共6页Chinese Journal of Tissue Engineering Research

摘　　要：背景:缺血再灌注后可以引起关节软骨的退行性变化,但其中具体机制尚无公识。目的:观察发育期髋关节股骨头骺关节软骨及缺血再灌注后关节软骨的退行性变化及其细胞凋亡现象。方法:80只SD大鼠随机分为2组,缺血再灌注组建立髋关节缺血再灌注实验动物模型,假手术组开腹暴露腹主动脉5min常规关腹。于模型建立后6h,12h,24h,48h,5d,2周,4周不同时点对股骨头骺关节软骨进行光镜病理形态学观察,同时对标本进行TUNEL法检测股骨头骺关节软骨细胞凋亡现象。结果与结论:缺血再灌注组术后光镜观察到软骨细胞变性、减少、基质可见局限性纤维化等关节软骨退行变化,两组TUNEL法检测均观察到关节软骨细胞的凋亡现象,假手术组偶见有散在分布5个以下的软骨细胞凋亡,缺血再灌注组观察到48h可见10-30个软骨细胞发生凋亡。结果提示,缺血再灌注对髋关节股骨头骺关节软骨可引起退行性改变,发育期髋关节缺血再灌注后关节软骨的细胞凋亡可能参与关节软骨的损害,抑制关节软骨的细胞凋亡可能有助于防治早发的骨关节炎。METHODS： A total of 80 Sprague-Dawley rats were randomly assigned to two groups： ischemia/reperfusion （model of ischemia/reperfusion in hip joint） and sham-surgery （exposure of abdominal aorta for 5 minutes） groups, with 40 animals in each group. Articular cartilages of femoral head epiphysis were collected in 6, 12, 24, and 48 hours, 5 days, and 2 and 4 weeks after operation. Morphology of articular cartilage of femoral head epiphyses was examined by light microscope, and cell apoptosis was detected by TUNEL method. RESULTS AND CONCLUSION： Light microscopy showed chondrocytes degeneration and reduction, as well as fibrosis in matrix of cartilage in the ischemia/reperfusion group. Chondrocyte apoptosis was observed in both groups by TUNEL. Several apoptotic cells, less than five, were observed in the sham-surgery, while 10-30 apoptotic cells were found in ischemia/reperfusion group at 48 hours. Results indicated that ischemia/reperfusion can induce degenerative changes in articular cartilage of femoral head epiphyses, and cell apoptosis in developing hip joint may participate in damage of articular cartilage. Inhibition of chondrocyte apoptosis in articular cartilage may be useful for the prevention and cure of early osteoarthritis.

关 键 词：组织构建 软骨组织构建 缺血再灌注 股骨头骺 髋关节 关节软骨 细胞凋亡 软骨细胞 动物模型 

分 类 号：R318[医药卫生—生物医学工程]