周期性张应力与软骨细胞基质金属蛋白酶的表达  

Cyclic tensile stress affects the expression of matrix metalloproteinases in chondrocytes

作　　者：刘兴漠[1] 孙青[1] 项禹诚[1] 梅新君[1] 黄胜[1] 潘滔[1] 

机构地区：[1]中山大学附属第六医院骨科,广东省广州市510655

出　　处：《中国组织工程研究》2013年第28期5157-5163,共7页Chinese Journal of Tissue Engineering Research

基　　金：广东省科技厅科技计划项目(2010B031100025);广东省医学科研基金资助项目(A2010204)~~

摘　　要：背景:课题组前期研究证实,在关节软骨缺损及骨性关节炎的动物模型中,软骨细胞可过度表达基质金属蛋白酶,各种异常刺激均可能打破基质金属蛋白酶与金属蛋白酶组织抑制因子间平衡,从而导致关节软骨细胞外基质退变,软骨细胞功能下降和失调。目的:观察兔关节软骨缺损修复过程中周期性张应力对软骨细胞基质金属蛋白酶表达的影响。方法:建立兔单侧膝关节软骨缺损模型,术后10周分离软骨细胞体外培养,非手术侧软骨细胞为正常组,术侧软骨细胞随机分为高应力组、低应力组及对照组,加载幅度为sin10%,0.1,1.0,0Hz的周期性张应力,于24h、48h、1周、2周和4周后RT-PCR测定各组基质金属蛋白酶2,3,9,13的表达。结果与结论:加载周期性张应力24h后,正常组及对照组间的基质金属蛋白酶2,3,9,13的表达差异有显著性意义(P<0.05);加载周期性张应力1周、2周及4周后高应力组和低压力组间差异有显著性意义(P<0.05);同时发现,低应力组中基质金属蛋白酶2,3,9,13的表达持续下降,加载周期性张应力24h与4周之间的差异有显著性意义(P<0.05)。可见力学载荷可影响兔关节软骨缺损修复过程中基质金属蛋白酶的表达,在细胞分子水平上,关节软骨缺损病理的发生发展与应力相互影响。METHODS： The animal models of articular cartilage defects were established, and chondrocytes were separated for culture at 10 weeks after operation. The chondrocytes on the non-surgical side were considered as the normal group and the chondrocytes on the surgical side were randomly divided into high cyclic tensile strain group, low cyclic tensile strains group and control group, and the load amplitude was sin10%. Then 0.1, 1.0 and 0 Hz cyclic tensile strains were loaded respectively. The expressions of matrix metalloproteinases 2, 3, 9 and 13 in each group were detected with reverse transcription-PCR at 24, 48 hours, 1,2 and 4 weeks after loading cyclic tensile strain. RESULTS AND CONCLUSION： There were significant differences in the expressions of matrix metalloproteinases 2, 3, 9 and 13 at 24 hours after loading cyclic tensile strain between the normal group and the control group （P 〈 0.05）; and there were significant differences in the expressions between the high cyclic tensile strain group and the low cyclic tensile strain group at 1, 2 and 4 weeks after loading cyclic tensile strain （P 〈 0.05）.At the same time, the expressions of matrix metalloproteinases 2, 3, 9 and 13 in the low cyclic tensile strain group were continued to decline, and there were significant differences in the expressions after Ioa^ding cyclic tensile strain for 24 hours and 4 weeks （P 〈 0.05）. The results indicate that mechanical load can affect the expression of matrix metalloproteinases in the healing process of rabbit articular cartilage defects, in the cellular and molecular level, the incidence and development of pathological articular cartilage defect and stress should affect each other.

关 键 词：组织构建 软骨组织构建 周期性张应力 软骨细胞 基质金属蛋白酶 应力 关节软骨缺损 基质金属蛋白酶2 基质金属蛋白酶3 基质金属蛋白酶9 基质金属蛋白酶13 省级基金 

分 类 号：R318[医药卫生—生物医学工程]