人胚肺成纤维细胞复制性衰老过程中端粒相关因子的表达  

Telomere-associated factor expression in replicative senescence of human embryonic lung fibroblasts

作　　者：杜华[1] 徐晓艳[1] 海玲[1] 师迎旭[2,3] 

机构地区：[1]内蒙古医科大学基础医学院病理解剖学教研室,内蒙古自治区呼和浩特市010059 [2]内蒙古医科大学附属医院临床研究中心,内蒙古自治区呼和浩特市010050 [3]中国医学科学院北京协和医学院血液学研究所,天津市300020

出　　处：《中国组织工程研究》2013年第28期5184-5190,共7页Chinese Journal of Tissue Engineering Research

基　　金：内蒙古医科大学资助项目(YKD2012KJBW006)~~

摘　　要：背景:端粒相关蛋白直接影响端粒的功能,调节端粒DNA的长度,与细胞的衰老和癌变密切相关。目的:通过观察正常细胞复制性衰老过程中端粒相关因子的变化来找寻细胞正常衰老过程中的关键调控分子。方法:在构建好的细胞复制性衰老模型基础上,利用RT-PCR与westernblot方法在分子与蛋白水平检测端粒相关因子的表达变化,主要检测人胚肺成纤维细胞在复制性衰老过程中端粒相关因子人端粒结合蛋白1、端锚聚合酶1、端粒酶RNA、端粒末端保护蛋白1以及P53的表达情况。结果与结论:结果显示,随着细胞的衰老,人端粒结合蛋白1的转录水平未发生改变,而人端粒结合蛋白1的蛋白表达水平有逐渐升高而后快速降低的过程;端锚聚合酶1的mRNA水平及蛋白表达水平未发生变化;端粒末端保护蛋白1随着人胚肺成纤维细胞的衰老表达水平逐渐降低;端粒酶RNA组分随着细胞的衰老呈递增趋势;P53的蛋白表达未发生变化。综上认为,人端粒结合蛋白1、端粒末端保护蛋白1及端粒酶RNA在细胞的复制性衰老过程中起重要作用。METHODS： Based on established cell replicative senescence model, reverse transcription-PCR and western blot were used to detect the telomere-associated factor expression on the molecular and protein levels, including the telomere-associated factor human telomere binding protein 1, tankyrase 1, telomerase RNA, telomere protection protein 1 and P53 expressions in the human embryonic lung fibroblast replicative senescence. RESULTS AND CONCLUSION： The results showed that with the cell senescence, transcription of human teiomere binding protein 1 did not changed, while the protein expression of human telomere binding protein 1 was increased gradually and then decreased rapidly; mRNA and protein expressions of telomere protection protein 1 did not changed; with the human embryonic lung fibroblast replicative senescence, expression of telomere protection protein 1 was decreased gradually; with ceil senescence, telomerase RNA component showed an increasing trend; protein expression of P53 did not changed. Human telomere binding protein 1, telomere protection protein 1 and telomerase RNA play an important role in cell senescence.

关 键 词：组织构建 组织构建与生物活性因子 复制性衰老 端粒结合蛋白1 端锚聚合酶1 端粒末端保护蛋白1 端粒酶RNA P53 其他基金 

分 类 号：R318[医药卫生—生物医学工程]