ALT作为血液质量判断指标的评价  

Evaluation of ALT as judgment index of blood quality

作　　者：漆萍[1] 钟方才 万小涛 邓文英[1] 吴垚林 

机构地区：[1]内江市中心血站 [2]内江第一人民医院,四川内江641000

出　　处：《川北医学院学报》2013年第4期385-387,共3页Journal of North Sichuan Medical College

摘　　要：目的:评价丙氨酸氨基转移酶(alanine aminotransferase,ALT)作为血液质量判断指标的价值。方法:对经过初筛合格且ALT>40U/L的标本使用ELISA方法检测HBsAg、抗-HCV,利用核酸扩增检测技术(nucleic Acid amplification tech-niques,NAT)对标本进行HBV-DNA和HCV-RNA的检测。结果:3 675份无偿献血者标本中有397份(10.80%)ALT>40U/L。在这397份标本中有389份(97.99%)的HBsAg和抗-HCV定性均阴性、HBV-DNA和HCV-RNA定量均呈现低拷贝数。而另8份(2.01%)标本的HBV-DNA或HCV-RNA呈现高拷贝数,其中3份标本HBsAg定性阳性、HBV-DNA呈现高拷贝数;4份标本抗-HCV定性阳性、HCV-RNA呈现高拷贝数;1份标本HBV-DNA呈现高拷贝数,HBsAg和抗-HCV定性阴性。结论:ALT作为判断血液质量的指标将造成大量血液资源的浪费。采用NAT作为判断血液质量指标,既能保证血液质量,又能保证血液不浪费。Objective：Evaluate ALT as judgment index of blood quality. Methods： All of the samples are qualified in preliminary screening and meet the requirement of ALT 〉 40U/L. ELISA were performed to test HBsAg, anti-HCV. NAT（nucleic acid amplifica- tion techniques） were used to detect HBV-DNA and HCV-RNA. Results：Among 3 675 voluntary blood donors, 397 （10. 80% ） sam- ples showed ALT 〉 40U/L. Among the 397 samples, 389 （97.99%） were of negative HBsAg and anti-HCV. The quantification of HBV-DNA and HCV-RNA showed low copy number. However, 8（2.01% ） samples＇ HBV-DbIA or HCV-RNA appeared high concen- tration. Three of them were of positive HBsAg and HBV-DNA presented high copy number. And 4 samples were anti-HCV positive and HCV-RNA presented high concentration. One sample＇s HBV-DNA was of high concentration, but HBsAg and anti-HCV were nega- tive. Conclusion：Using ALT as judgment index of blood quality will waste a large number of blood resources. Using NAT as judgment index of blood quality can ensure the quality of blood and reduce the waste of blood resources.

关 键 词：丙氨酸氨基转移酶 酶联免疫吸附试验 核酸检测技术 血液质量 血液资源 

分 类 号：R446.1[医药卫生—诊断学] Q555.6[医药卫生—临床医学]