骨髓间充质干细胞及与胰岛细胞共移植治疗糖尿病  

Co-transplantation of bone marrow mesenchymal stem cells and islet cells in the treatment of diabetes mellitus

作　　者：吴立萍[1] 李蒙[2] 李瑞玉[3] 孙艳浮 颜军礼 

机构地区：[1]邢台市人民医院检验科,河北省邢台市054031 [2]武警新疆总队和田地区支队卫生队,新疆维吾尔自治区和田市848011 [3]邢台医学高等专科学校第二附属医院中西医结合研究所,河北省邢台市054000 [4]河北省任县医院,河北省邢台市055150

出　　处：《中国组织工程研究》2013年第31期5711-5716,共6页Chinese Journal of Tissue Engineering Research

基　　金：河北省中医药管理局资助项目(2012068)~~

摘　　要：背景:药物是目前糖尿病治疗最主要的方法,但病情的进展以及相关并发症的发生使药物的作用受到挑战。目的:探讨骨髓间充质干细胞与胰岛细胞共移植治疗糖尿病的应用效果以及可行性。方法:分离、纯化大鼠骨髓间充质干细胞并进行体外培养,建立糖尿病模型,对糖尿病模型大鼠分别注射骨髓间充质干细胞、骨髓间充质干细胞和胰岛细胞共培养混合物以及生理盐水或磷酸盐缓冲液作为对照。通过观察监测各糖尿病模型大鼠血糖水平的变化、胰岛素分泌情况以及胰腺组织的病理学变化评估移植治疗效果。结果与结论:骨髓间充质干细胞移植治疗糖尿病模型大鼠,移植后C-肽值明显升高,血糖水平明显下降,但仍未降至正常范围内,且随着时间的延长,血糖水平再次升高。骨髓间充质干细胞与胰岛细胞共移植治疗糖尿病模型大鼠,血糖水平亦明显下降,且下降程度大于单纯骨髓间充质干细胞移植治疗,可下降至正常水平,并在一定时间内能够维持。骨髓间充质干细胞与胰岛细胞共移植治疗糖尿病具有一定的效果,具有应用的可行性。BACKGROUND： Drug treatment is the main method for the treatment of diabetes currently, but the development of the disease and occurrence of related complications are the challenges to the effect of drugs. OBJECTIVE： To investigate the effect and feasibility of co-transplantation of bone marrow mesenchymal stem cells and islet cells for the treatment of diabetes. METHODS： The rat bone marrow mesenchymal stem cells were separated and purified, and cultured in vitro to establish the diabetes models. The rat diabetes models were injected with bone marrow mesenchymal stem cells, co-cultured mixture of bone marrow mesenchymal stem cells and islet cells, and normal saline or phosphate buffer （control）. The effect of transplantation was evaluated through observing the blood glucose levels, insulin secretion, and pathological changes of pancreatic tissue in the rat diabetes models. RESUTLS AND CONCLUSION： In the diabetes rats treated with bone marrow mesenchymal stem cell transplantation, the C-peptide levels were significantly increased after transplantation, while the blood glucose levels were significantly decreased, but not lower than the normal level, and the blood glucose levels were increased again with the time prolonging. In the diabetes rats treated with co-transplantation of bone marrow mesenchymal stem cells and islet cells, the blood glucose levels were decreased significantly and lower than the normal level which was maintained in a certain time, and the decreasing degree was larger than that in the rats treated with simple bone marrow mesenchymal stem cell transplantation. Co-transplantation of bone marrow mesenchymal stem cells and islet cells is feasible for the treatment of diabetes with a certain effect.

关 键 词：器官移植 器官移植学术探讨 骨髓间充质干细胞 胰岛细胞 糖尿病 移植 胰腺 血糖 胰岛素 免疫荧光染色 省级基金 

分 类 号：R318[医药卫生—生物医学工程]