A comparative study of proliferation and osteogenic differentiation of rat adipose-derived stem cells in β-tricalcium phosphate (β-TCP), forsterite (Mg_2SiO_4 ) and clinoenstatite (MgSiO_3)  

作　　者：LENG Bin JIN XiaoGang LIN QiuXia CHEN Lei WANG Yan DU ZhiYan LIN KaiLi CHANG Jiang GU XiaoMing WANG ChangYong 

机构地区：[1]Department of Implantology of People's Armed Police General Hospital [2]Department of Advanced Interdisciplinary Studies, Institute of Basic Medical Sciences and Tissue Engineering Research Center, Academy of Military Medical Sciences [3]State Key Laboratory of High Performance Ceramics and Superfine Microstructure, Shanghai Institute of Ceramics, Chinese Academy of Sciences

出　　处：《Chinese Science Bulletin》2013年第24期3033-3042,共10页

基　　金：supported by the National Natural Science Foundation of China (30730034);the National Basic Research Program of China(2011CB606206)

摘　　要：In this study, the effects of forsterite and clinoenstatite powder extracts on the proliferation and osteogenic differentiation of rat adipose-derived stem cells (ASCs) were investigated and compared with the β-tricalcium phosphate (β-TCP) powder extracts. Methylthiazolyldiphenyl-tetrazolium bromide (MTT) assay and live-dead staining were performed to evaluate the viability and proliferation of rat ASCs. Osteogenic differentiation of rat ASCs were assayed by alkaline phosphatase (ALP) staining and ALP activity test. The expression of osteogenic marker genes (alkaline phosphatase (ALP), runt related transcription factor 2 (Runx2), collagen type Iα1 (Col1α1), secreted phosphoprotein1 (Spp1, osteopontin), integrin binding sialoprotein (Ibsp), bone gla protein (Bglap)) were detected by real-time polymerase chain reaction (PCR). The MTT assay and the live-dead staining showed that all the three ceramics possessed good cytocompatibility with rat ASCs. Furthermore, forsterite and clinoenstatite promoted the proliferation of rat ASCs compared with β-TCP. The results of the ALP activity test and the real-time PCR demonstrated that forsterite and clinoenstatite promoted the osteogenic differentiation of rat ASCs. These results suggested that forsterite and clinoenstatite are bioactive ceramics that may be used for preparation of bone tissue engineering (BTE) scaffolds.In this study, the effects of forsterite and clinoenstatite powder extracts on the proliferation and osteogenic differentiation of rat adipose-derived stem cells （ASCs） were investigated and compared with the β-tricalcium phosphate （β-TCP） powder extracts. Methylthiazolyldiphenyl-tetrazolium bromide （MTT） assay and live-dead staining were performed to evaluate the viability and proliferation of rat ASCs. Osteogenic differentiation of rat ASCs were assayed by alkaline phosphatase （ALP） staining and ALP activity test. The expression of osteogenic marker genes （alkaline phosphatase （ALP）, runt related transcription factor 2 （Runx2）, collagen type Iα1 （Collα1）, secreted phosphoproteinl （Sppl, osteopontin）, integrin binding sialoprotein （lbsp）, bone gla protein （Bglap）） were detected by real-time polymerase chain reaction （PCR）. The MTT assay and the live-dead staining showed that all the three ceramics possessed good cytocompatibility with rat ASCs. Furthermore, forsterite and clinoenstatite promoted the pro- liferation of rat ASCs compared with β-TCP. The results of the ALP activity test and the real-time PCR demonstrated that forster- ite and clinoenstatite promoted the osteogenic differentiation of rat ASCs. These results suggested that forsterite and clinoenstatite are bioactive ceramics that may be used for preparation of bone tissue engineering （BTE） scaffolds.

关 键 词：脂肪干细胞 成骨分化 细胞增殖 镁橄榄石 TCP相 磷酸三钙 Mg2SiO4 MgSiO3 

分 类 号：R329[医药卫生—人体解剖和组织胚胎学]