新疆地区烟粉虱生物型的区域分布及其携带的番茄黄化曲叶病毒检测  

Biotype distribution and infection status of tomato yellow leaf curl virus (TYLCV) in Bemisia tabaci ( Hemiptera:Aleyrodidae) in Xinjiang, northwestern China

作　　者：曹骞[1] 李晶 买热木古丽.克依木 王惠卿 李国志[1] 马德英[1,3] 

机构地区：[1]新疆农业大学农学院,乌鲁木齐830052 [2]新疆维吾尔自治区植物保护站,乌鲁木齐830006 [3]新疆农业大学设施农业研究所,乌鲁木齐830052

出　　处：《昆虫学报》2013年第6期652-664,共13页Acta Entomologica Sinica

基　　金：新疆维吾尔自治区科技计划项目(201231116)

摘　　要：【目的】明确不同烟粉虱Bemisia tabaci(Gennadius)生物型在新疆地区的区域分布及其携带番茄黄化曲叶病毒的情况。【方法】本文利用烟粉虱mtDNA COⅠ基因片段作标记以及番茄黄化曲叶病毒(tomato yellow leaf curlvirus,TYLCV)特异性引物,鉴定新疆不同地区烟粉虱生物型,并检测其携带TYLCV情况。【结果】石河子市、博乐市、岳普湖县、泽普县、叶城县、莎车县、墨玉县、和田市与和田县烟粉虱属于B型烟粉虱,且720bp COⅠ基因序列一致性为100%;阿克苏市、哈密市、鄯善县、乌鲁木齐市、克拉玛依市与伊宁市烟粉虱属于Q型烟粉虱,720bp COⅠ基因序列一致性为99.9%～100%;吐鲁番市与库尔勒市兼有B型烟粉虱和Q型烟粉虱。伊宁市、乌鲁木齐市、鄯善县、哈密市、阿克苏市、库尔勒市、莎车县、和田县和墨玉县的烟粉虱样本携带TYLCV,除2011年底已经暴发危害的莎车县外,其他区域均为番茄黄化曲叶病毒病发生的高风险区域。【结论】本研究为新疆地区烟粉虱及番茄黄化曲叶病毒病的科学防控提供依据。【Aim】 The purpose of the paper is to determine the distribution of Bemisia tabaci biotypes and their infection status of tomato yellow leaf curl virus （TYLCV） in Xinjiang, northwestern China. 【Methods】 Mitochondrial cytochrome oxidase I （mtDNA COⅠ） gene sequences and two pairs of primers （TYLCVF/TYLCV-R and PA/PB） were utilized to identify biotypes of B. tabaci and to detect the status of carrying tomato yellow leaf curl virus （TYLCV）. 【Results】 The results showed that samples collected from Shihezi city, Bole city, Yopurga county, Zepu county, Karghalik county, Shache county, Karakash county, Hotan city and Hotan county belong to the B biotype, and these samples showed 100% identity of the 720 bp COⅠ sequences examined with the references. Samples from Aksu city, Hami city, Shanshan county, Urumqi city, Karamay city and Yining city belong to the Q biotype, and their COⅠ sequence identities were 99.9%-100%. Both B biotype and Q biotype occurred in Turpan and Korla cities. TYLCV were detected in samples collected from Yining city, Urumqi city, Shanshan county, Hami county, Aksu city, Korla city, Shache county, Hotan county and Karakash county, and all these areas were in high risk of TYLCV occurrence except Shache county where TYLCV had already broken out in late 2011. 【Conclusion】 This study provides scientific basis for the control of Bemisia tabaci and the outbreak of TYLCV in Xinjiang.

关 键 词：烟粉虱 生物型 MTDNA COⅠ基因 番茄黄化曲叶病毒 新疆 

分 类 号：Q965.8[生物学—昆虫学]