环磷酰胺处理致小鼠睾丸支持细胞去分化抑制精子发生  

Cyclophosphamide-induced dedifferentiation of sertolic cells inhibit spermatogenesis in mouse

作　　者：王庆忠[1] 王汉海[1] 刘慧莲[1] 王东方[1] 冯道俊[1] 刘世武[1] 

机构地区：[1]山东省高校生物化学与分子生物学重点实验室潍坊学院生物与农业工程学院,山东潍坊261061

出　　处：《毒理学杂志》2013年第3期176-179,共4页Journal of Toxicology

基　　金：山东省自然科学基金(ZR2010CL015);潍坊市科技发展计划(2011022;20111023;2010143);潍坊学院优秀学术团队项目(2010Z03)

摘　　要：目的研究环磷酰胺对小鼠支持细胞的影响。方法睾丸组织切片的苏木素-伊红(HE)染色观察环磷酰胺对睾丸支持细胞的影响,通过免疫组织化学和细胞化学分析检测支持细胞中CK-18等的表达,通过Western-blot法检测ERK1/2的表达。结果环磷酰胺处理导致小鼠支持细胞凋亡和数量减少,残存的支持细胞去分化,去分化的支持细胞中的ERK1/2-MAPK信号通路被激活。结论环磷酰胺处理活化了ERK1/2-MAPK信号通路并诱导支持细胞去分化。Objective To study the effects of cyclophosphamide on Sertoli cells. Methods The effects of cyclophosphamide on Sertoli cells were observed by HE staining of mouse testis slices, the CK-18 expressions were analyzed by immunocytochemistry and immunohistochemistry, and the expressions of ERK1/2 in testis after cyclophosphamide treatment were detected by western-bloting. Results Cyclophosphamide treatment induced the apoptosis of Sertoli cells and cut down the number of Sertoli cells in testis, the remnants of Sertoli cells in mouse testis dedifferentiated, and the ERK1/2-MAPK signaling pathway was activated. Conclusion The cyclophosphamide treatment activated the ERK1/2-MAPK pathway and induced the dedifferentiation of Sertoli cells.

关 键 词：环磷酰胺 支持细胞 去分化 小鼠 

分 类 号：Q492[生物学—生理学]