季铵盐联吡啶-钌(Ⅱ)配合物的合成及其与小牛胸腺DNA的相互作用  

Synthesis of quaternary ammonium 2,2′-dipyridyl derivativeRu(Ⅱ)complex and its interaction with calf thymus DNA

作　　者：陈嘉曦[1] 郝洪庆[2] 黄大坤 黄丹梅[1] 林海玲[1] 孙静[1] 

机构地区：[1]广东医学院药学院,广东东莞523808 [2]嘉应学院化学与环境学院,广东梅州514015

出　　处：《化学研究》2013年第4期394-398,402,共6页Chemical Research

基　　金：国家自然科学基金(21101034;21101077);东莞市高等院校科研机构科技项目(2011108102046);湛江市科技攻关计划项目(2011C3107019;2011C3107023);广东医学院大学生创新课题(2011ZZDM001)基金资助项目

摘　　要：利用紫外-可见吸收光谱、荧光光谱、圆二色谱等光谱手段,以及黏度测定等流体力学方法在三羟甲基氨基甲烷(tris)缓冲溶液和磷酸缓冲溶液中研究了含季铵盐联吡啶配体的钌(Ⅱ)配合物[Ru(L)(phen)2](PF6)4(L=5,5′-二(三甲铵基甲基)-2,2′-联吡啶离子,phen=邻菲咯啉)与小牛胸腺DNA(CT-DNA)的相互作用.结果表明,合成的配合物与CT-DNA之间存在一定的亲和作用,拟合得到的结合常数可达105;与此同时,该配合物能够稳定CT-DNA的结构,并对CT-DNA有较好的立体选择性(右旋异构体优先与DNA结合).The interaction between culf thymus DNA（CT-DNA）and Ru（Ⅱ）complex[Ru（L）（phen）2]（PF6）4（L = 5,5′-di（trimethylammoniomethyl）-2,2′-dipyridyl cation,phen= 1,10phenanthroline）was studied by means of ultraviolet-visible light absorption spectrometry,fluorescence spectrometry,and circular dichroism spectrometry as well as viscosity measurements in tris（hydroxymethyl）aminomethane（denoted as tris）buffer and phosphoric acid buffer.Results show that there exist a certain degree of affinity between as-synthesized Ru（Ⅱ）complex and CT-DNA,and the intrinsic binding constant obtained by fitting is as much as 105.In the meantime,the Ru（Ⅱ）complex can induce the stabilization of CT-DNA and possesses good steric selectivity towards CT-DNA（its right-handed enantiomer is preferentially bound to CTDNA.

关 键 词：季铵盐联吡啶 钌(Ⅱ)配合物 合成 小牛胸腺DNA 相互作用 

分 类 号：O614.82[理学—无机化学]