固体表面延迟荧光法测定中草药中有效成分柚皮甙  

Solid Surface Delayed Fluorometry for Determination of an Effective Constituent of Chinese Herbal Medicine Naringin

作　　者：尚晓虹[1] 董川[2] 刘长松[2] 

机构地区：[1]山西医科大学,太原030006 [2]山西大学化学系,太原030006

出　　处：《分析试验室》2000年第5期24-26,共3页Chinese Journal of Analysis Laboratory

基　　金：国家自然科学基金!(295752 04);省青年科学基金!981006资助课题

摘　　要：以层析聚酰胺膜为基质 ,以 L i Ac作增强剂 ,建立了测定痕量中草药有效成分柚皮甙的固体表面延迟荧光法。该法取样量少 (2 μL) ,线性范围宽 (2 .9～ 580 .0 ng/斑 ) ,检出限为 0 .0Solid surface delayed fluorometry(SS DF) for determination of naringin using polyamide membrane as substrate was described. Various effect factors on SS DF intensity such as substrate, pH condition,drying temperature were studied in detail. The excitation wavelengths were 298nm (weak) and 380nm(strong),the emission wavelengths were 450nm and 480nm(shoulder peak).The linear dynamic range (LDR) is 2.9～580.0 ng/spot,and the detection limit is 0.033ng/spot.

关 键 词：固体表面荧光法 延迟荧光 柚皮甙 中草药 测定 

分 类 号：R284.1[医药卫生—中药学] O657.3[医药卫生—中医学]