萘降解菌的分离鉴定以及脱氢酶活性的测定  

Isolation and Identification of Naphthalene-degrading Strains and Determination of Dehydrogenase Activity

作　　者：徐建红[1] 李日强[1] 李红娇[1] 杨喜[1] 

机构地区：[1]山西大学环境与资源学院,山西太原030006

出　　处：《山西大学学报（自然科学版）》2013年第3期471-474,共4页Journal of Shanxi University(Natural Science Edition)

基　　金：山西省科技攻关资助项目(20100321024)

摘　　要：利用焦化废水中含有的污染物萘为唯一碳源,从处理焦化废水的活性污泥中分离能降解萘的细菌菌株,采用GenIII MicroStatioTM型BIOLOG微生物自动鉴定仪对其进行鉴定,并采用三苯基甲臜(TF)定量分析法测其脱氢酶活性.结果表明,根据菌落特征分离出3株生长良好的细菌菌株NDS1、NDS2和NDS3,经鉴定分别为Pseudo-monas alcaligenes、Pseudomonas viridilivida和Pseudomonas resinovorans,脱氢酶活性分别为10.315μg·(mL·h)-1、6.630μg.(mL·h)-1和9.175μg·(mL·h)-1.Using naphthalene which was pollutant of coking wastewater as the only carbon source,naphthalene-degrading strains was isolated and breed from cativated sludge of coking-plant wastewater.They were identified by MicroStationTM BIOLOG Identification System.The triphenyl formazan（TF） quantitative analysis method was used to determine dehydrogenase activity of naphthalene-degrading strains..They turned out that they were isolated three strains of bacteria NDS1,NDS2 and NDS3 which were identified as Pseudomonas alcaligenes,Pseudomonas viridilivida and Pseudomonas resinovorans.Dehydrogenase activity of naphthalene-degrading strains were respectively 10.315 μg·（mL·h）-1,6.630 μg·（mL·h）-1 and 9.175 μg·（mL·h）-1.

关 键 词：焦化废水 萘 分离 鉴定 脱氢酶活性 

分 类 号：Q93-331[生物学—微生物学] X78[环境科学与工程—环境工程]