桑树绿枝扦插生根过程中乙烯合成相关基因aco和sams的表达分析  

Expression Analysis of Ethylene-synthesis Related Genes aco and sams During the Rooting Process of Mulberry Softwood Cuttings

作　　者：聂浩[1] 焦锋[2] 张晓峰[1] 李小玉[1] 唐壮[1] 程嘉翎[1,3] 

机构地区：[1]江苏科技大学,江苏镇江212003 [2]西北农林科技大学,陕西杨凌712100 [3]中国农业科学院蚕业研究所,江苏镇江212018

出　　处：《蚕业科学》2013年第4期633-637,共5页ACTA SERICOLOGICA SINICA

基　　金：现代农业产业技术体系专项(No.CARS-22)

摘　　要：植物内源乙烯对愈伤组织的诱导、增殖,不定芽、不定根的形成以及体细胞胚的诱导等过程都会产生重要的影响。利用荧光定量PCR技术检测诱导桑树绿枝插穗基部皮层生根过程中与乙烯合成相关的1-氨基-羧酸环丙烷氧化酶基因aco和S-腺苷-L-蛋氨酸合成酶基因sams的转录水平变化。结果表明插穗生根发育过程中aco和sams均表现为表达下调,其中生根率低的未诱导对照组插穗中这2个基因的转录水平均表现出先小幅下降后升高的变化特点,而生根率高的诱导组插穗中这2个基因则表现为持续低水平转录,尤其在插穗生根发育后期,2个基因在2个试验组插穗中的转录水平变化呈现近乎相反的变化特点。研究结果初步说明,乙烯合成相关基因aco和sams的mRNA转录水平与桑树绿枝插穗的生根率呈现负相关性。Plant endogenous ethylene has an important influence on induction and proliferation of callus,formation of adventitious bud and adventitious root,and induction of somatic embryo.Transcriptional levels of two ethylene-synthesis related genes,aminocyclopropane-1-carboxylate oxidase gene（aco） and S-adenosyl-L-methionine systhetase gene（sams）,in basal cortex of mulberry softwood cuttings during the rooting process were measured by real-time fluorescent quantitative PCR.The results indicated that the expressions of aco and sams were both down-regulated during the rooting process of cuttings.The transcriptional levels of these two genes in cuttings of the control group with low rooting rate showed a first decreasing slightly and then increasing tendency.Those in the treatment group with high rooting rate were expressed continuously at low level.Especially at the late developmental stage of rooting,transcriptional level variation of the two genes showed a nearly opposite pattern.It was preliminarily illustrated that the mRNA transcriptional levels of ethylene-synthesis related genes aco and sams are negatively correlated with rooting rate of mulberry softwood cuttings.

关 键 词：桑树 扦插 生根率 内源乙烯 1-氨基-羧酸环丙烷氧化酶基因 S-腺苷-L-蛋氨酸合成酶基因 实时荧光定量PCR 

分 类 号：S888[农业科学—特种经济动物饲养]