家蚕氨肽酶N家族基因在5龄幼虫中肠组织的表达分析  

Expression Analysis of Aminopeptidase N Family Genes in Midgut Tissue of the Fifth Instar Bombyx mori Larvae

作　　者：王猛[1] 程晨[1] 郝碧芳[1,2] 徐安英[1,2] 沈兴家[1,2] 黄金山[1,2] 

机构地区：[1]江苏科技大学,江苏镇江212003 [2]中国农业科学院蚕业研究所,江苏镇江212018

出　　处：《蚕业科学》2013年第4期689-694,共6页ACTA SERICOLOGICA SINICA

基　　金：国家重点基础研究发展计划"973"项目(No.2012CB1146-04);江苏省自然科学基金项目(No.BK2011525);江苏省教育厅科技项目(No.11KJB180002)

摘　　要：氨肽酶N(aminopeptidase N,APN)是一种偏好水解蛋白质或寡肽N端中性氨基酸的酶,在鳞翅目昆虫中主要分布于中肠上皮细胞的刷状缘囊膜上,是苏云金芽孢杆菌(Bacillus thuringiensis,Bt)伴孢晶体(Cry)毒素的重要受体蛋白。为了研究家蚕APN家族基因的表达特征,运用Real-time PCR技术检测分析该家族基因在不同家蚕品种幼虫中肠组织的表达差异以及同一家蚕品种幼虫中肠组织中该家族基因各个成员的表达丰度。在所有供试家蚕品种的幼虫中肠组织均可检测到APN家族基因的表达,但不同化性家蚕品种间APN基因的表达水平存在显著差异(P<0.05),而相同化性品种间的差异较小(P>0.05);在同一家蚕品种幼虫中肠组织中,APN2、APN4、APN5基因的表达量较高,而APN1及APN3基因的表达量较低。研究结果有助于进一步研究家蚕APN家族基因的作用机制,并为家蚕抗Bt品种的选育提供理论依据。Aminopeptidase N（APN） is an enzyme that readily hydrolyzes protein or neutral amino acids at the N-terminal of an oligopeptide.It is mainly distributed in the brush border membrane vesicle of midgut epithelial cells of lepidopterous insects and is the important receptor of Bacillus thuringiensis crystal toxins（Cry）.To investigate expression patterns of APN family genes,Real-time PCR was employed to analyze the differential expression of APN family genes in larva migut tissue of different silkworm（Bombyx mori） varieties and the expression level of various family members in larva midgut tissue of the same silkworm variety.The results showed that APN family genes were expressed in larva midgut of all tested silkworm varieties.The expression level of APN genes were significantly different between silkworm varieties having different voltinisms（P＆lt;0.05）.However,there was little difference between silkworm varieties having the same voltinism（P＆gt;0.05）.The expression levels of APN 2,APN 4 and APN 5 genes were relatively higher in larva midgut tissue of the same silkworm variety,whereas those of APN 1 and APN 3 were relatively lower.The obtained results would facilitate further study on functioning mechanism of silkworm APN family genes,and provide theoretical basis for breeding silkworm strains resistant to Bacillus thuringiensis.

关 键 词：家蚕 氨肽酶N基因 中肠组织 荧光定量PCR 品种 化性 

分 类 号：S881.2[农业科学—特种经济动物饲养]