同步检测胆囊及Oddi氏括约肌电活动和胆管压力的兔模型  

A rabbit model for simultaneous recording the electromyography of the gallbladder and sphincter of Oddi and the pressure of gallbladder and common bile duct

作　　者：文辉清[1] 沈磊[1] 时鑫 龙萍[1] 刘衍民[1] 

机构地区：[1]广州医学院第一附属医院普通外科,510230 [2]云南曲靖第一人民医院微创外科

出　　处：《中华实验外科杂志》2013年第8期1767-1768,共2页Chinese Journal of Experimental Surgery

摘　　要：目的建立同步检测胆囊及Oddi括约肌肌电活动及胆囊、胆总管压力的动物模型。方法对6只家兔用7F静脉深穿管分别经肝穿刺进入胆囊腔及经十二指肠腔插入胆总管。将铂金电极缝在胆囊底部浆膜上。以多通道生理仪记录家兔的胆囊及Oddi括约肌肌电信号和胆囊、胆总管压力。结果Oddi氏括约肌肌电的峰电位〉0．05mV，最大电位为0．26mV，频率为0-2次／分；而慢波电位≤0．05mV，频率为8-11次／分。胆囊内压力最大为15emH．0（6．83-15．00emH2O），胆总管最大压力为22cm H2O（11～22emH2O）。胆囊肌电活动的波形缺乏规律性。结论兔可以作为同步检测胆囊及Oddi氏括约肌肌电活动和胆囊、胆总管压力的模型。Objective To investigate an animal model for recording the electromyography （EMG） of the gallbladder/sphincter of Oddi and the pressure of gallbladder and common bile duct. Methods The animal model was established in 6 rabbits. A 7F central venous catheter was adopted to penetrate into the gallbladder through the liver. A pair bipolar platinum electrode was stitched onto the gallbladder at the se- rosal side. Another 7F central venous catheter with a pair bipolar platinum electrode was used to insert into the common bile duct near the sphincter of Oddi （SO）. The signal of myoelectrical activity of SO and the gallbladder was recorded through a multi-channel physiological instrument. Meanwhile the changes in the pressure of the gallbladder and common bile duct were observed. Results The spike potential of SO in rab- bits was above 0. 05 mV, and the most value was 0. 26 mV. The frequency of the spike potential was 2 times/min. The slow wave potential of SO was below 0. 05 mV, and the frequency was 8-11 times/min. The pressure of gallbladder was 15 cm H2O （6. 83-15.00 cm H20）. The pressure of common bile duct was 22 cm H2O （ 11-22 cm H2 O）. The EMG of gallbladder was unclear. Conclusion Rabbits can be used to establish the animal model recording the EMG of gall bladder and SO, and the pressure of common bile duct and gallbladder simultaneously, which provides a method for studying the myoelectric activity of SO and gallbladder motility.

关 键 词：胆囊 ODDI氏括约肌 肌电活动 兔 

分 类 号：R657.4[医药卫生—外科学]