聚苯胺用作乙醇脱氢反应中的电子传递介质  

Polyaniline Used as An Electron Transfer Mediator for the Catalyzed Dehydrogenating Reaction of Alcohol

作　　者：穆绍林[1] 杨一飞[1] 

机构地区：[1]扬州大学理学院化学系,扬州225002

出　　处：《物理化学学报》2000年第9期830-834,共5页Acta Physico-Chimica Sinica

基　　金：国家自然科学基金

摘　　要：The aniline solution containing nicotinamide adenine dinucleotide(NAD＋ )was electrolyzed. Polyaniline film with NAD＋ formed on the platinum thin plate. The thickness of the film was controlled by the charge consumed in the electrolysis, which was 0.006 C in this case. This electrode, used as an anode, was immersed in the solution containing aniline.A second film formed on this electrode during the electrolysis. The charge passed during the second electrolysis was 0.016 C. Alcohol dehydrogenase(ADH) was immobilized on the polyaniline film based on the isoelectric point of ADH and the doping principle of conducting polymers. The enzyme electrode has a good bioelectrochemical response to ethanol in the absence of a homogeneous mediator. The optimum pH, apparent Michaelis- Menten constant and optimum temperature of ADH immobilized in polyaniline film are 7.83, 7.5 mmol· dm－ 3, and 30.6 ℃ , respectively.The activation energy of the enzyme- catalyzed reaction is 33.5 kJ· mol－ 1. The response current of the enzyme electrode increases linearly with increasing concentration of ethanol below 2.4 mmol· dm－ 3.This enzyme electrode can be used to determine the concentration of ethanol.The aniline solution containing nicotinamide adenine dinucleotide(NAD＋ )was electrolyzed. Polyaniline film with NAD＋ formed on the platinum thin plate. The thickness of the film was controlled by the charge consumed in the electrolysis, which was 0.006 C in this case. This electrode, used as an anode, was immersed in the solution containing aniline.A second film formed on this electrode during the electrolysis. The charge passed during the second electrolysis was 0.016 C. Alcohol dehydrogenase(ADH) was immobilized on the polyaniline film based on the isoelectric point of ADH and the doping principle of conducting polymers. The enzyme electrode has a good bioelectrochemical response to ethanol in the absence of a homogeneous mediator. The optimum pH, apparent Michaelis- Menten constant and optimum temperature of ADH immobilized in polyaniline film are 7.83, 7.5 mmol· dm－ 3, and 30.6 ℃ , respectively.The activation energy of the enzyme- catalyzed reaction is 33.5 kJ· mol－ 1. The response current of the enzyme electrode increases linearly with increasing concentration of ethanol below 2.4 mmol· dm－ 3.This enzyme electrode can be used to determine the concentration of ethanol.

关 键 词：脱氢酶聚苯胺 电子传递介质 乙醇 脱氢反应 

分 类 号：O621[理学—有机化学]