非灰髓炎肠道病毒与婴幼儿急性下呼吸道感染关系的研究  

A study on the relationship between non-polio enteroviruses infection and acute lower respiratory infections in infants and young children

作　　者：罗荣[1] 董永绥[2] 方峰[2] 李革[2] 

机构地区：[1]湖北省十堰市人民医院,442000 [2]同济医科大学附属同济医院儿科教研室

出　　处：《中国实用儿科杂志》2000年第8期471-473,共3页Chinese Journal of Practical Pediatrics

摘　　要：目的　了解非灰髓炎肠道病毒 (EV)感染在婴幼儿急性下呼吸道感染 (ALRI)中的发病情况、临床特点及诊断方法。方法　①采用通用引物RT -PCR及病毒分离检测全年住院ALRI婴幼儿鼻咽分泌物 (NPS)中的EV。②并用APAAP法检测RSV、IFV、PIV和AdV ,以资比较。结果　①观察组 87例NPS中RT -PCR阳性 40例 ,EV分离阳性 2 6例。②阳性病例中同时检出其它呼吸道病毒 2 6例。结论　①近半数住院ALRI婴幼儿存在EV感染 ,且常与其它呼吸道病毒混合感染。②EV -RT -PCR较EV分离敏感、实用。Objective To investigate the incidence,clinical features and diagnostic methods of non-polio enterovirus(EV) infection in acute lower respiratory infection in infants and young children.Methods ①EV from nasopharygeal secretions(NPS) was detected by RT-PCR and virus isolation in 87 in-patients with ALRI.②RSV,IFV,PIV and ADV were detected by enzyme immunohistochemistry assay.Results ①40 out of 87 patients had EV infection in RT-PCR and 26 in EV isolation.②26 patients were found to be coinfected with other respiratory viruses.Conclusion ①nearly half of the patients with ALRI had EV infection and often coinfected with the other respiratory viruses.②EV-RT-PCR is more sensitive and practical than EV isolation.③EV infection has no clinical specificity.

关 键 词：非灰髓炎肠道病毒 婴幼儿 下呼吸道感染 PT-PCR 

分 类 号：R725.602[医药卫生—儿科]