混合酶强化剩余污泥微生物燃料电池性能  

Enhanced performance of surplus sludge microbial fuel cells by additional mixed enzymes

作　　者：张植平[1,2,3] 刘志华[1,2] 李小明[1,2,4] 杨麒[1,2] 罗琨[1,2] 杨慧[1,2] 王子龙[1,2] 

机构地区：[1]湖南大学环境科学与工程学院,长沙410082 [2]环境生物与控制教育部重点实验室(湖南大学),长沙410082 [3]湖南艾布鲁环保科技有限公司,长沙410007 [4]广西大学环境学院,南宁530004

出　　处：《环境工程学报》2013年第8期3209-3215,共7页Chinese Journal of Environmental Engineering

基　　金：国家自然科学基金资助项目(51078128);国际科技合作项目(2011DFA90740);湖南省科技计划重点项目(2010WK2012)

摘　　要：为了提高剩余污泥为燃料的微生物燃料电池(SMFC)产电性能以及污泥减量化效果,在不同的温度(40、45和50℃)研究单室无膜微生物燃料电池中酶对SMFC产电特性的强化效果。加入单一酶(蛋白酶或α-淀粉酶)的结果表明,随着温度的上升,SMFC功率密度均上升,但40℃时强化效果最明显,与加入失活酶的对照组相比分别增加198%和130%。在40℃下,混合酶比(蛋白酶浓度:淀粉酶浓度)为2∶3时,SMFC最大功率密度为776 mW/m2。随着混合酶中淀粉酶的比例提高,SMFC库伦效率逐渐增加,当混合酶比为4∶1时,CE(库伦效率)可达18.3%,同时TCOD、TSS和VSS去除率分别为70.3%、66.7%和80.4%。因此,温度相对较低时,外加酶强化效果更明显;与单种酶相比,混合酶对SMFC产电性能和污泥减量化的强化效果更显著。In order to improve electricity production and sludge reduction in surplus sludge microbial fuel cells（SMFC）,enhanced effect of enzyme on membrane-free single-chamber SMFC was investigated at different temperatures（40,45 and 50℃）.The results showed that the power density of SMFC increased with the increase of temperature for both neutral protease and α-amylase addition.The most significant enhanced effect of enzymes was obtained at 40℃.Compared with the control group（added inactivated enzyme）,the power density increased 198% and 130%,respectively.Moreover,the effect of different ratio of enzymes（protease/amylase） on electricity production and sludge reduction in SMFC was analyzed at 40℃.The maximum power density of MFC was 776 mW/m2 when the ratio of enzyme was 2/3.The higher the proportion of amylase,the bigger the battery coulomb efficiency was obtained,which could reach 18.3% when the ratio of enzyme was 4/1.Correspondingly,the removal rates of TCOD,TSS and VSS were 70.3%,66.7% and 80.4%,respectively.According to aforementioned,the enhanced effect of enzyme was more obvious when the temperature was relatively low.Compared with the single enzyme,the mix enzyme could strengthen the electrical production and the reduction of sludge in single chamber SMFC.

关 键 词：剩余污泥 混合酶 微生物燃料电池 功率密度 减量化 

分 类 号：X703.5[环境科学与工程—环境工程]