应用A组乙型链球菌特异性菌膜抗原诊断风湿性心脏炎的探讨  

Gruop a streptococcal membrance used as a specific antigen of diagnosis of rheumatic carditis

作　　者：余步云[1] 张汉伟[1] 古洁若[1] 张韶冈[1] 

机构地区：[1]中山医科大学附属第三医院风湿科,广州510630

出　　处：《风湿病学杂志》2000年第4期206-208,共3页

基　　金：卫生部临床学科重点基金资助项目![2 7( 0 70 ) ]

摘　　要：目的　研究A组乙型溶血性链球菌菌膜抗原在外周血淋巴细胞促凝血活性试验中诊断风湿性心脏炎的特异性。方法　分别用A组乙型溶血性链球菌菌膜抗原和 1%植物血凝素(PHA)作为刺激物 ,分别测定 38名健康对照组 ,30例风湿性心脏病活动组 (风心活动组 ) ,2 0例风湿性心脏病静止组 (风心静止组 )外周血淋巴细胞促凝血活性 (procoagulantactivity ,PCA)。 结果　在特异性菌膜抗原刺激下 ,风心活动组PCA (18 1± 6 3) %显著高于风心静止组 (7 2± 1 9) %和健康对照组 (6 0± 3 0 ) % ,而后二者无统计学差异。在受PHA刺激后 ,风心活动组PCA (9 0± 2 5 ) % ,风心静止组 (6 2± 1 6 ) % ,和健康对照组 (7 5± 3 2 ) %三者差异无显著性。结论　以链球菌菌膜为促凝刺激物的PCA试验在判断风湿性心脏炎方面具有特异性 ,其特异性取决于所用的刺激原为特异性抗原。Objective To research the specificity of antigen from group A streptococcal cell membrane in procoagulant activity assay for the diagnosis of rheumatic carditis.Method A specific antigen from group A streptococcal cell membrane and 1%PHA were used as stimulants to test the peripheral blood lymphocyte procoagulant activity (PCA) in 30 cases of active rheumatic heart disease,20 cases of inactive rheumatic heart diseases and 38 cases of normal control.Results When streptococcal cell membrane was used as a stimulant,PCA of active rheumatic heart disease group (18 1±6 3)% was much higher than that of inactive rheumatic heart disease group (7 2±1 9)% and normal control group (6 0±3 0)%.It was found that no statistical difference between the latter 2 groups (P>0 05),While 1% PHA was used as stimulant,there was no statistical difference among the three groups (P>0 05),PCA in active,inactive and normal control groups was (9 0±2 5)%,(6 2±1 6)% and (7 5±3 2)% respectively.Conclusion PCA is of specific significance for detecting the activity of rheumatic carditis and its specificity is based on the specific antigen from groups A streptococal membrane.

关 键 词：风湿性心脏病 A组乙型链球菌 特异性菌膜抗原 

分 类 号：R541.2[医药卫生—心血管疾病]