淋巴细胞对系统性红斑狼疮患者粒-巨噬细胞集落形成单位抑制活性的研究  

Lymphocyte depression to systemic lupus erythematosus patient′s granulocyte-macrophage colony forming unit

作　　者：李娟[1] 尹培达[2] 罗绍凯[1] 彭爱华[1] 

机构地区：[1]中山医科大学附属第一医院血液科,广州510080 [2]中山医科大学附属第一医院风湿科,广州510080

出　　处：《风湿病学杂志》2000年第4期209-212,共4页

基　　金：广东省科委重点攻关基金资助项目!( 982 782 0 )

摘　　要：目的　探讨系统性红斑狼疮 (SLE)患者B、T淋巴细胞对SLE及正常人骨髓粒巨噬细胞集落形成单位 (GM CFU)的影响。方法　分别用SLE患者及正常人B、T及B +T淋巴细胞加入半固体甲基纤维素培养体系中 ,观察它们分别对 16例SLE患者及 12名正常人GM CFU产率的影响。结果　加入SLE患者B、T淋巴细胞后 ,分别可见 75 0 %和 37 5 %的SLE患者GM CFU形成减少 ,产率为 (10 9± 71)个 /10 5细胞和 (137± 34 )个 /10 5细胞 ,与未加淋巴细胞组比较差异均有显著性(P <0 0 1,P <0 0 5 ) ,同时加入SLE患者B和T淋巴细胞时 87 5 %的SLE患者GM CFU产率减少 ,为 (98± 31)个 /10 5细胞 ,与分别加入SLE患者B或T淋巴细胞组比较均有明显差异 (P <0 0 5 ,P <0 0 1) ;SLE患者B及B +T淋巴细胞分别对 5 8 4%、6 6 7%的正常人骨髓GM CFU形成有抑制作用 ,产率为 (2 0 3± 6 7)、(199± 92 )个 /10 5细胞 ,明显低于未加淋巴细胞组 (P均 <0 0 1) ,而正常人淋巴细胞对SLE患者GM CFU的产率与未加淋巴细胞组比较差异无显著性 (P均 >0 0 1)。结论　SLE患者B淋巴细胞无论对大部分SLE患者或大部分正常人骨髓GM CFU的形成有抑制作用 ,SLE患者的B +T淋巴细胞对SLE患者和正常人骨髓GM CFU的抑制有协同作用 。Objective To research the influence of B lymphocytes,T lymphocytes on systemic lupus erythematosus (SLE) patient′s and control′s granulocyte macrophage colony forming unit (GM CFU).Methods GM CFU of 16 active SLE patients and 12 controls was cultured and observed in vitro in semisolid methylcellulose with B lymphocytes and T lymphocytes respectively and together.Result Decreasing of GM CFU appeared in 75 0% or 37 5% of SLE patients after SLE B lymphocytes or T lymphocytes added to culture system.The products were (109±71) colonies/10 5 cells and (137±34) colonies/10 5 cells respectively,differed from nonlymphocyte SLE patient′s ones (P<0 01,P<0 05).The product which was (98±31) colonies/10 5 cells in 87 5% of patients in culture system adding with B and T lymphocytes was lower than B or T lymphocytes groups (P<0 05,P<0 01).Reduction of GM CFU appeared in 58 4% and 66 7% of controls with B lymphocyte and B lymphocyte + T lymphocyte in SLE,respectively.The puoducts were (203±67) colonies/10 5 cells and (199±92) colonies/10 5 cells which were all lower than nonlymphocyte one (P<0 01).The products of SLE patients cultured with controls lymphocyte had not different from nonlymphocyte ones (P>0 01).Conclusion SLE patient′s B lymphocyte may restrain GM CFU of not only most control′s but also most SLE patient′s.B and T lymphocyte in SLE have the effect of cooperation with restraining the products of GM CFU of SLE patients or controls.Abnormality of B lymphocyte in SLE plays a main role in leukopenia.Abnormality of hematopoietic stem cell may be also one cause of leukopenia.

关 键 词：系统性红斑狼疮 淋巴细胞 GM-CFU B细胞 T细胞 

分 类 号：R593.24[医药卫生—内科学]