频谱多普勒超声检测大鼠肝缺血/再灌注后入肝血流量变化与血清TNF-α及IL-1β水平变化之间的关系  

Relationship Between The Changes of Hepatic Blood Flow Detected by Using Spectral Doppler Ultrasound and Serum TNF-α and IL-1 β Levels after Liver Ischemia/Reperfusion of Rat

作　　者：张云飞[1] 阙艳红[1] 王学梅[1] 李弘[2] 代娣[3] 李晓姝[1] 

机构地区：[1]中国医科大学附属第一医院超声科,辽宁沈阳110001 [2]中国医科大学附属第一医院普通外科教研室器官移植科,辽宁沈阳110001 [3]中国医科大学附属第一医院检验科,辽宁沈阳110001

出　　处：《中国普外基础与临床杂志》2013年第7期742-745,共4页Chinese Journal of Bases and Clinics In General Surgery

基　　金：沈阳市科学技术计划项目(项目编号:F10-149-9-53)~~

摘　　要：目的探讨频谱多普勒超声检测大鼠肝缺血/再灌注(I/R)后入肝血流量变化与血清肿瘤坏死因子-α(TNF-α)及白介素-1β(IL-1β)水平变化之间的关系。方法 Pringle法建立肝脏缺血15 min再灌注模型,应用频谱多普勒超声检测再灌注后1、6及24 h不同时间点肝动脉及门静脉的入肝血流量,计算总血流量(FV),观测肝I/R后入肝血流量的变化情况。检测各时间点血清TNF-α及IL-1β水平的变化,分析FV与TNF-α及IL-1β之间的相关性。结果 I/R组再灌注后1及6 h的FV分别为(52.08±11.88)mL/min及(44.69±8.75)mL/min,较假手术组术后1及6 h的(85.32±29.85)mL/min和(81.41±28.67)mL/min明显减少(P<0.05);再灌注后24 h FV 2组间的差异无统计学意义(P>0.05)。I/R组再灌注后1 h血清TNF-α含量为(310.52±39.83)pg/mL,较假手术组术后1 h的(240.74±31.65)pg/mL高(P<0.05);再灌注或手术后6及24 h的TNF-α含量2组间差异无统计学意义(P>0.05)。I/R组再灌注后1及6 h血清IL-1β含量分别为(38.08±3.73)pg/mL和(27.44±6.11)pg/mL,较假手术组术后1和6 h组的(22.03±0.79)pg/mL及(21.78±0.71)pg/mL高(P<0.01,P<0.05);再灌注后24 h的血清IL-1β含量2组间差异无统计学意义(P>0.05)。FV与TNF-α及IL-1β之间存在负相关关系(r=-0.43,P<0.05;r=-0.46,P<0.05)。结论频谱多普勒超声能够通过检测入肝血流量的变化间接判断肝脏微循环状态,肝I/R后入肝血流量减少,且血流量的减少可能与TNF-α和IL-1β的过表达有关。Objective To discuss the relationship between the changes of hepatic blood flow detected by using spectral Doppler ultrasound and serum TNF-α and IL-1 β levels after liver ischemia/reperfusion（I/R）of rat.Methods The hepatic ischemia 15 min and reperfusion models were established by using pringle method.The hepatic blood flow of hepatic artery and portal vein at 1,6,and 24 hours after liver I/R were detected by using spectral Doppler ultrasound,the total blood flow volume（FV）was calculated,and the serum TNF-α and IL-1 β levels at each time point were detected.The correlation between the TNF-α,IL-1 β,and FV were analyzed.Results The FV at 1 hour and 6 hours after reperfusion in I/R group were less than those in sham operation（SO）group〔（52.08±11.88）mL/min vs.（85.32±29.85）mL/min and（44.69±8.75）mL/min vs.（81.41±28.67）mL/min,P0.05〕.The FV at 24 hours after operation or reperfusion of 2 groups was no significant differences（P 0.05）.The serum content of TNF-α at 1 hour after reperfusion in I/R group was higher than that in SO group〔（310.52±39.83）pg/mL vs.（240.74±31.65）pg/mL,P0.05〕.The serum contents of TNF-α at 6 and 24 hours after operation or reperfusion of 2 groups were no significant differences（P0.05）.The serum contents of IL-1β at 1 hour and 6 hours in I/R group were higher than those in SO group〔（38.08±3.73）pg/mL vs.（22.03±0.79）pg/mL and（27.44±6.11）pg/mL vs.（21.78±0.71）pg/mL,P0.05〕.The serum content of IL-1β at 24 hours after operation or reperfusion of 2 groups was no significant differences（P0.05）.There was a negative correlation between the FV and TNF-α or IL-1β（r =-0.43,P0.05;r =-0.46,P0.05）.Conclusions Spectral Doppler ultrasound can observe the changes of hepatic blood flow and evaluate the hepatic microcirculation indirectly.The hepatic blood flow after liver I/R decreases and it may be related to over expression of TNF-α and IL-1β.

关 键 词：肝 缺血 再灌注 频谱多普勒超声 肿瘤坏死因子-α 白细胞介素-1β 

分 类 号：R445.1[医药卫生—影像医学与核医学] R657.3[医药卫生—诊断学]