小麦生理型雄性不育系中反式烯脂酰-CoA还原酶基因(TaECR)的克隆及表达分析  

Cloning and Expression Characteristic of Trans-2,3-enoyl-CoA Reductase Gene(TaECR) in Physiological Male Sterile Line of Wheat (Triticum aestivum)

作　　者：巴青松[1] 张改生[1] 车会学[1] 刘红占[1] 张龙雨[1] 王书平[1] 

机构地区：[1]西北农林科技大学,国家杨凌农业生物技术育种中心/国家小麦改良中心杨凌分中心/小麦育种教育部工程研究中心/陕西省作物杂种优势研究与利用重点实验室,杨凌712100

出　　处：《农业生物技术学报》2013年第7期792-798,共7页Journal of Agricultural Biotechnology

基　　金：国家高技术研究发展计划(863)项目(No.2011AA10A106);国家自然科学基金项目(No.31071477;31171611);高等学校博士学科点专项科研基金(No.20090204110024);陕西省"13115"科技创新工程重大科技专项(No.2010ZDKG-68;2011KTZB02-01-01)

摘　　要：超长链脂肪酸(VLCFAs)代谢在花药发育中发挥着重要的作用,而反式烯脂酰-CoA还原酶(trans-2,3-enoyl-CoA reductase,ECR)是催化超长链脂肪酸合成的脂肪酰-CoA延长酶之一。为进一步研究杀雄剂SQ-1诱导小麦雄性不育的机理,根据已报道二穗短柄草(Brachypodium distachyon)ECR基因,采用电子克隆获得序列并设计引物,从小麦(Triticum aestivum)中克隆ECR的开放阅读框cDNA序列,命名为TaECR,将该序列提交至GenBank,登录号为KC222053。序列分析表明,TaECR基因编码310个氨基酸,具有反式烯脂酰-CoA还原酶的经典结构域。表达分析表明,TaECR基因在小麦花药、颖片、叶和根中均有表达,其中在根中的表达量最底;与可育系相比,TaECR基因在生理型不育系三核期中表达急剧下调,在单核期和二核期表达趋势无显著变化,并与其蜡质积累量变化趋势基本一致,这表明TaECR基因调节的超长链脂肪合成途径可能参与了SQ-1诱导的败育过程。Very-long-chain fatty acids（VLCFAs） play an important role in anther development. VLCFAs is catalyzed by the fatty acyl-CoA elongase, a membrane-bound enzymatic complex containing trans-2,3-enoyl- CoA reductase（ECR）. To further study the mechanism of male sterility induced by gametocide SQ- 1 in wheat （Triticum aestivum L.）, primers were designed based on silicon cloning sequence of ECR gene sequences from Brachypodium distachyon, and the open reading frame of the cDNA were obtained, here as designated TaECR that had been registered under GenBank（No. KC222053）.Sequence analysis showed the open reading frame of this gene putatively encoding 310 amino acids, and had classical domains of ECR. Analysis showed that 7）tECR was ubiquitously expressed in anther, glume, leaf and root in wheat, but it was less expressed in root. Compared with fertile lines, the expression levels of TaECR in the physiological male sterile line were much lower than those in fertile line in trinucleate stage, but it showed no significant difference in unicleate and binucleate stage, which very corresponded to trends of wax loads in the development of the anther. These results suggest that the pathway of very long chain fatty synthetic metabolism regulated by TaECR gene may participate in abortion process of sterile line induced by SQ-1.

关 键 词：小麦 杀雄剂SQ-1 TaECR 超长链脂肪酸 雄性不育 

分 类 号：S512.1[农业科学—作物学]