中国明对虾WSSV携带量、生长和抗WSSV育种值的相关性分析  

Correlation analysis of the viral loads with growth and white spot syndrome virus resistance breeding value of Fenneropenaeus chinensis

作　　者：肖广侠[1,2] 孔杰[1] 孟宪红[1] 罗坤[1] 栾生[1] 曹宝祥[1] 刘宁[1] 

机构地区：[1]中国水产科学研究院黄海水产研究所,山东青岛266071 [2]天津渤海水产研究所,天津300457

出　　处：《水产学报》2013年第7期1009-1014,共6页Journal of Fisheries of China

基　　金：国家自然科学基金项目(31072206;31172402);青岛市关键技术攻关类项目(11-1-1-11-h);国家农业科技成果转化资金项目(2011GB2A210003)

摘　　要：为评估中国明对虾生长及抗WSSV能力与中国明对虾WSSV携带量之间的相关性,实验对130个中国明对虾家系进行生长及抗WSSV性能测试,对收集到的中国明对虾生长和抗病数据,输入"水产动物育种分析与管理系统"数据库,利用综合选择指数法估计中国明对虾各个家系的育种值。根据分析结果,选择出生长育种值最大的5个家系和最小的5个家系、抗WSSV育种值最大的5个家系和最小的5个家系,分别检测上述20个家系的亲虾、养殖50 d及170 d中国明对虾的WSSV携带量。结果显示,亲虾、50 d中国明对虾及170 d中国明对虾的WSSV携带量分别为0.190 8、0.286 6和0.232 9 copies/ng DNA,三者之间差异均不显著。亲虾、50 d中国明对虾和170 d中国明对虾的WSSV携带量与中国明对虾的生长育种值相关系数分别为0.021、0.363和0.185,亲虾、50 d中国明对虾和170 d中国明对虾的WSSV携带量与抗WSSV育种值相关系数分别为0.033、0.048和0.019。研究表明,中国明对虾的生长育种值和抗WSSV育种值与中国明对虾体内的WSSV携带量均无显著的相关性。The epidemic pathogen of Fenneropenaeus chinensis caused by white spot syndrome virus （WSSV） have brought great loss of the F. chinensis cultivation, no effective technical measures could control the WSSV epidemic so far. Improving the germplasm resources and cultivating the new varieties of F. chinensis are better measures to deal with this disease. The correlation among F. chinensis traits is an important parameter, as a result, it is very important to choose an appropriate genetic evaluation method in the analysis of correlation among F. chinensis traits. After testing the growth and white spot syndrome virus （WSSV） resistance of 130 F. chinensis families, we collected the F. chinensis growth and resistance data, and entered the date into database of aquatic animal breeding analysis and management software. According to the results of statistics software analysis, using the comprehensive index method to estimate growth and WSSV resistance breeding value of each F. chinensis family, we chose the five families each of the largest and the smallest growth breededing value, five families each of largest and smallest WSSV-resistance breeding value. We detected the WSSV load of these broodstocks, 50 d prawn and 170 d prawn of 20 families. The mean WSSV load of broodstock,50 d prawn and 170 d prawn of 20 families were 0. 190 8, 0. 286 6 and 0. 232 9 copies/ng DNA,respectively, which showed no significant difference （ P 〉 0.05 ）. The correlation coefficients between WSSV-resistance breeding value and WSSV load of broodstock,50 d prawn and 170 d prawn were 0. 021,0. 463 and 0. 185 ,respectively;and those between growth breeding value and WSSV load of broodstock,50 d shrimp and 170 d shrimp were 0. 033,0. 048 and 0. 019,respectively. The results show that the relationships among growth breeding value, WSSV-resistance breeding value and the WSSV load of shrimp were not significant（P 〉 0.05 ）.

关 键 词：中国明对虾 生长 白斑综合征病毒 育种值 相关性 

分 类 号：Q348[生物学—遗传学] S917.4[农业科学—水产科学]