广东一株重组H9N2亚型禽流感病毒的鉴定及全基因组序列分析  

Complete genomic evolutionary analysis of a reassortant H9N2 influenza virus isolated from poultry flocks in Guangdong

作　　者：申翰钦[1] 曾凡桂[2] 严专强[1] 刘佳佳[1] 覃健萍[2] 毕英佐[1] 李海燕[2] 陈峰[1] 

机构地区：[1]华南农业大学动物科学学院,广东广州510642 [2]广东温氏食品集团股份有限公司广东温氏集团研究院,广东云浮527439

出　　处：《中国预防兽医学报》2013年第8期607-612,共6页Chinese Journal of Preventive Veterinary Medicine

基　　金：国家自然科学基金(31072152)

摘　　要：本研究于2013年从广东某发病鸡场分离到一株H9N2亚型AIV,命名为A/chicken/Guangdong/LG1/2013。对病毒进行全基因组克隆测序和进化分析,其HA基因裂解基序为333PSRSSR↓GLF341,呈典型低致病性AIV分子特征。其HA基因发生Q226L突变和A316S突变,NA基因出现第63～65位氨基酸缺失。该病毒株HA、NA和NS基因属于类BJ/1/94分支,PB2属于类SD/H/09分支,PB1、PA、NP基因属于类SH/F/98分支,M基因属于类HK/G1/97分支,该基因型在广东地区未见报道,其内部基因与H7N9亚型人流感病毒SH/02/2013株核苷酸同源性在95.6%～98.6%之间。本研究表明我国H9N2亚型AIV呈现遗传演化的多样性及基因重组的复杂性,对该亚型病毒的监测和研究具有重要的兽医和公共卫生意义。To investigate the genetic and evolutionary characteristics of H9N2 avian influenza virus （A1V） in poultry flocks in Guangdong province, an H9N2 A1V was isolated and designated A/chicken/Guangdong/LG1/2013 （CK/GD/LG1/13）. The phylogenetic and molecular characteristics of the isolate were analyzed. The HA cleavage sequence of the isolate possessed the 333pSRSSR ↓ GLF341 motif, suggesting that this isolate was of a low pathogenicity in avian species. However, this isolate had an A316S mutation at the HA cleavage site and a 3-amino-acid deletion at position 63 to 65 on the NA stalk, which indicated an enhanced virulence in chickens and mice. Furthermore, CK/GD/LG1/13 also had the Q^6L mutation at HA receptor-binding site which could increase the binding of the virus to human-type receptors, SAct-2, 6. The HA, NA and NS genes of the isolate belong to BJ/1/94-1ike lineage. The PB2 belongs to H-like and the M gene belongs to Gl-like lineage, while the PBI, PA and NP genes belong to F98-1ike lineage. The genotype of the isolate in poultry flocks in Guangdong province has never been reported before. It is noteworthy that six internal genes of the isolate share 95.6%-98.6% nucleotide identities with that of SH/1/2013 （H7N9）. The findings of mammalian-adapting mutations and probable efficient replication in mammalians in this novel AIV are caused for concem a novel benefit. Ke This study demonstrated that H9N2 virus in south China had undergone extensive evolution and reassortment to generate viruses possesses significant veterinary and public health

关 键 词：禽流感病毒 H9N2亚型 全基因组 基因特征 

分 类 号：S852.65[农业科学—基础兽医学]