Quantitative analysis of cefixime via complexation with palladium(Ⅱ) in pharmaceutical formulations by spectrophotometry  

作　　者：Syed Najmul Hejaz Azmi Bashir Iqbal Nada Said Hassan Al-Humaimi Iman Rashid Saif Al-Salmani Noora Ali Saad Al-Ghafri Nafisur Rahman 

机构地区：[1]Department of Applied Sciences,Chemistry Section,Higher College of Technology [2]Department of Chemistry,Aligarh Muslim University

出　　处：《Journal of Pharmaceutical Analysis》2013年第4期248-256,共9页药物分析学报（英文版）

摘　　要：An optimized and validated spectrophotometric method has been developed for the determination of cefixime in pharmaceutical formulations. The method is based on the complexa- tion reaction between cefixime and palladium ion in the presence of acidic buffer solution （pH 3） in ethanol-distilled water medium at room temperature. The complex absorbed maximally at 352 nm. Beer＇s law is obeyed in the working concentration range of 2.5-35 μg/mL with apparent molar absorptivity of 1.015×104 L/mol cm and Sandell＇s sensitivity of 0.001 μg/cm2/0.001 absorbance unit. The limits of detection and quantitation for the proposed method are 0.175 and 0.583μg/mL, respectively. The effect of common excipients used as additives has been studied in the determination of cefixime. The proposed method has been successfully applied for the determina- tion of cefixime in pharmaceutical formulations. The results obtained by the proposed method were statistically compared with the reference method using t and F values and found no significant difference between the two methods.An optimized and validated spectrophotometric method has been developed for the determination of cefixime in pharmaceutical formulations. The method is based on the complexa- tion reaction between cefixime and palladium ion in the presence of acidic buffer solution （pH 3） in ethanol-distilled water medium at room temperature. The complex absorbed maximally at 352 nm. Beer＇s law is obeyed in the working concentration range of 2.5-35 μg/mL with apparent molar absorptivity of 1.015×104 L/mol cm and Sandell＇s sensitivity of 0.001 μg/cm2/0.001 absorbance unit. The limits of detection and quantitation for the proposed method are 0.175 and 0.583μg/mL, respectively. The effect of common excipients used as additives has been studied in the determination of cefixime. The proposed method has been successfully applied for the determina- tion of cefixime in pharmaceutical formulations. The results obtained by the proposed method were statistically compared with the reference method using t and F values and found no significant difference between the two methods.

关 键 词：SPECTROPHOTOMETRY CEFIXIME Palladium ion Pd（II）-cefixime complex 

分 类 号：R927[医药卫生—药学]