紫外-可见分光光度法测定巴豆中可溶性蛋白的含量  

Soluble Protein Content in Croton TigliumL with Ultraviolet-Visible Spectrophotometry

作　　者：古炳明[1] 曾宝[1] 熊艺花[1,2] 易智彪[1] 蔡海群[1] 肖祖平[1] 袁捷[1] 

机构地区：[1]广州中医药大学,510006 [2]广州中医药大学中医药数理工程研究院,东莞523808

出　　处：《医学研究杂志》2013年第8期43-45,共3页Journal of Medical Research

基　　金：国家科技部重大新药创制专项基金资助项目(2009ZX09103-388);广东省科技计划基金资助项目(2009A030100014)

摘　　要：目的建立巴豆中可溶性蛋白含量的测定方法,为其质量评价提供理论依据。方法采用紫外-可见分光光度(UV-VIS)法,检测波长为593nm。结果巴豆中可溶性蛋白在0.0103～0.1030mg/ml范围内线性关系良好(Y=8.0871X+0.1775,r=0.9991,n=6),平均回收率为98.37%,RSD为1.01%。结论本方法简单、方便、专属性强,可为巴豆药材的质量控制提供参考。Objective Ultraviolet- Visible Spectrophotometry was established to determine the Soluble protein content in Croton tiglium L. Methods Reference standard method （ k = 593nm） was used to determine the content of Soluble protein in Croton tiglium L. Results The test of Soluble protein in croton tiglium L. was linear within the range of 0. 0103 - 0. 1030mg/ml（ Y = 8. 0871X ＋ 0. 1775, r = 0. 9991 ,n = 6）. The average recovery was 98.37% , RSD = 1.01%. Conclusion The method was simple and accurate. It can pro- vide reference for croton tiglium L. materials quality control.

关 键 词：巴豆 紫外-可见分光光度法 可溶性蛋白 

分 类 号：R996.3[医药卫生—毒理学]