下咽壁工程化咽缩肌的形态观察与功能研究  

Morphology and Function of Engineered Pharyngeal Constrictor

作　　者：郭珊珊[1] 沈志森[2] 陈静静[1] 康骋[1] 裘世杰[2] 叶栋[2] 任远[2] 

机构地区：[1]宁波大学医学院,315211 [2]宁波市医疗中心李惠利医院,315400

出　　处：《医学研究杂志》2013年第8期88-91,共4页Journal of Medical Research

基　　金：宁波市重大(重点)课题(2008C50019);宁波市重大课题(2012C5015);宁波市科技创新团队(2012B82019)

摘　　要：目的本实验通过检测成肌细胞在不同的空间结构和不同蛋白表面改性的聚氨酯上的黏附、增殖和分化情况,比较不同聚氨酯薄膜的细胞相容性,希望为聚氨酯作为骨骼肌组织工程支架材料的可行性提供依据。方法制备不同的聚氨酯薄膜,对其进行性能检测并将其与从人的咽缩肌提取和体外培养的第3代成肌细胞共培养。采用MTT法、扫描电镜和Western blot检测成肌细胞在不同的支架上的黏附和生长情况。结果成肌细胞在三维的聚氨酯支架上呈现明显的极性分布。经丝素蛋白表面改性的聚氨酯薄膜力学性能好,黏附细胞数量多且细胞大量表达特异性肌源调节因子(MyoD)。其他各组细胞少,肌源性调节因子表达少。结论聚氨酯的生物力学性能良好,适用于骨骼肌组织工程。丝素蛋白作为一种天然的纤维蛋白,可以促进成肌细胞的增殖和分化。Objective This experiment is aimed to test the adhesion, proliferation and differentiation ability of myoblast on polyure- thane with different spatial structure and different surface modification. There is feasibility of polyurethane applied in the skeletal muscle tissue engineering as the result of cell compatibility of different scaffolds. Methods The characterizations of different polyurethane films were detected, and were cultured with myoblast which extracted from human pharyngeal constrictors. The adhesion and growth of myoblast on different scaffolds were valued by MTT method, scanning electron microscopy （SEM） and Western blot detection. Results Myoblasts on 3D polyurethane rendered polarity distribution obviously. Polyurethane membrane modified with silk fibroin had a better mechanical performance, more cell adhesion and MyoD expression. Conclusion Polyurethane is applicable to the skeletal muscle tissue engineering for its good biomechanical properties. Silk fibroin, as a natural protein fiber, can promote the proliferation and differentiation of myoblasts.

关 键 词：聚氨酯 丝素蛋白 咽缩肌 成肌细胞 三维支架 

分 类 号：R329-33[医药卫生—人体解剖和组织胚胎学]