miR-193b对子宫内膜癌侵袭的影响  

Effects of miR-193b on Endometrial Carcinoma Cell Invasion Potential

作　　者：刘颖[1] 朱月华[1] 曹卉[1] 

机构地区：[1]中国人民解放军第九七医院妇产科,徐州221004

出　　处：《医学研究杂志》2013年第8期145-148,共4页Journal of Medical Research

摘　　要：目的探讨miR-193b对人子宫内膜癌细胞侵袭的影响及其机制。方法人子宫内膜癌细胞HEC-1A细胞转染miR-193b抑制剂(miR-193b inhibitor)24h后,利用Transwell小室评价分析其侵袭能力的变化。进一步,我们利用Westernblot验证侵袭相关基质金属蛋白酶(matrix metalloprotease,MMP)-2、MMP-9蛋白的表达。结果 miR-193b inhibitor有效抑制HEC-1A细胞细胞内miR-193b表达,并且其侵袭能力较对照组明显下降(P<0.05)。Western blot验证miR-193b inhibitor可有效抑制MMP-2、MMP-9蛋白表达。结论 miR-193b通过可调节侵袭相关MMP-2、MMP-9蛋白表达有效调控HEC-1A细胞细胞侵袭能力。Objective To investigate the effect of miR - 193b on endometrial carcinoma cell invasion potential, and explore its pos- sible targets and mechanism. Methods Human endometrial carcinoma HEC - 1A cells were transfected with miR - 193b inhibitor. The change of invasion ability in HEC - 1A cells was measured by Transwell assay. Then the expressing levels of cell invasion related matrix metalloprotease （MMP） -2 and MMP -9 were identified by Western blot. Results miR - 193b inhibitor could effectively inhibit the expression of miR - 193b in HEC - 1A cells. And the invasive ability of cells transfected with miR - 193b inhibitor was obviouslyreduced. Western blot identified miR -193b inhibitor could effectively inhibit the expression of MMP -2 and MMP -9. Conclusion miR -193b could effectively regulate the HEC -1A cell invasion by regulating the expression of MMP- 2 and MMP- 9.

关 键 词：miR-193b 子宫内膜癌 侵袭 

分 类 号：R339.22[医药卫生—人体生理学]