北京市HIV-1 B’／C重组型分子流行特征分析  

Molecular epidemiological characteristics of HIV-1 B＇/C strains in Beijing

作　　者：叶景荣[1] 董晓根[2] 卢红艳[1] 郝明强[1] 苏雪丽[1] 辛若雷[1] 余双庆[4] 冯霞[4] 闫红[3] 贺雄[1] 曾毅[4] 

机构地区：[1]北京市疾病预防控制中心, 100013 [2]北京市丰台区疾病预防控制中心,100013 [3]北京工业大学生命科学与生物工程学院,100013 [4]中国疾病预防控制中心病毒病预防控制所

出　　处：《中华医学杂志》2013年第29期2301-2304,共4页National Medical Journal of China

摘　　要：目的分析北京市HIV-1B’／C重组型毒株的分子流行特征。方法采集北京市2006-2010年新发现B’／C重组型HIV-1感染者的抗凝全血标本，分离血浆和提取病毒RNA，用反转录／巢式聚合酶链式反应扩增病毒gag基因，并进行系统进化分析。结果成功扩增了159例标本，CRF07-BC147例，CRF08-BC12例，CRF07-BC系统进化树中存在3个主要的亚簇，IDU-Max亚簇包含89例样本，静脉吸毒传播占71．9％，同参考株97CN001组间离散率为2．8％；IDU-Min亚簇包含22例样本，静脉吸毒传播占77．3％，组间离散率为2．8％；MSM亚簇包含34例样本，男男同性传播占67．6％，组间离散率为3．1％；3个亚簇内均无其他国家的序列，只有IDU—Max亚簇包含1条来自中国台湾的序列；核苷酸多态性分析结果显示，以IDU．Max亚簇为参照，IDU．Min和MSM亚簇分别有32和41个位点的核苷酸组成存在著性差异。结论首次观察到北京市流行的CRF07-BC病毒株中存在3个独立的亚簇，异性传播和静脉吸毒传播序列混杂分布，而男同传播病例则聚集成1个亚簇，应当继续对B’／C重组型分子流行特征进行监测。Objective To analyze the molecular epidemiological characteristic of HIV-1 B＇/C strains prevalent in Beijing. Methods Plasma samples were collected from 200 newly diagnosed HIV-1 B＇/ C individuals reported during 2006 to 2010 in Beijing. The gag gene fragments were amplified from RNA template extracted from plasma with reverse transcription （RT） and nested polymerase chain reaction （PCR）. And the sequences were analyzed by phylogenetic methods and Entropy analysis. Results A total of 159 sequences were successfully amplified from the gag genes of which 147 was CRF07 BC and 12 CRF08_ BC. There were 3 main subclusters in CRF07_BC phylogenetie tree and they were named as subcluster IDU- Max （89 sequences）, subcluster IDU-Min （22 sequences） and subcluster MSM （34 sequences） based on transmission. No international reference strain was closely related with these three subclusters except for one strain identified in Taiwan. All CRF07_BC recombinant strains were remarkable for their low interpatient diversity in gag genes （3.7%, 3.3% and 2.0% for isolates from IDU-Max, IDU-Min and MSM respectively）. When compared with subcluster IDU-Max, there were 32 and 41 significantly different sites of nucleotide polymorphism compositions in subclusers IDU-Min and MSM. Conclusion This is the first report of describing the existence of three main epidemic subclusters in CRF07_BC strains prevalent in Beijing. And IDU-Max subeluster is the dominant strain. The CRF07_BC in Beijing are less diverse than other strains and may be derived from a common ancestor.

关 键 词：HIV 分子流行病学 巢式聚合酶链式反应 

分 类 号：R373[医药卫生—病原生物学]