伽玛刀治疗吗啡依赖猴双侧伏隔核后多巴胺D3受体变化的研究  

Change of Dopamine D3 Receptor Expression in Nucleus Accumbens after Gamma-knife Treatment on Morphine Dependent Rhesus Monkey

作　　者：黄晓斌[1] 赵宁辉[1] 曹洪明[2] 孙亮[2] 方绍龙[1] 

机构地区：[1]昆明医科大学第二附属医院神经外科,云南昆明650101 [2]昆明医科大学第二附属医院伽玛刀治疗中心,云南昆明650101

出　　处：《昆明医科大学学报》2013年第6期30-32,共3页Journal of Kunming Medical University

基　　金：云南省中青年学术技术带头人后备人才基金资助项目(2009CI034);云南省卫生厅卫生系统学科带头人基金资助项目(D-201221)

摘　　要：目的建立猴吗啡依赖实验模型,观察伏隔核、海马、扣带回、额叶皮质等脑区多巴胺D3受体成瘾后和治疗前后的变化.方法健康恒河猴4只,分为吗啡依赖伽玛刀治疗组(2只,A组)、吗啡依赖对照组(1只,B组)和正常对照组(1只,C组).模仿Seevers法剂量递增建立猴吗啡依赖模型,免疫组化法观察3组猴大脑标本伏隔核、海马、扣带回、额叶皮质中多巴胺D3受体的变化.结果免疫组化法检测发现大脑伏隔核、海马、扣带回、额叶皮质多巴胺D3受体表达B组明显高于C组(P<0.01),表达密度上调254%～499%不等,A组明显低于B组(P<0.01),但仍高于C组,其中伏隔核、海马、扣带回下降幅度较大,伏隔核较接近正常.结论伽玛刀治疗吗啡依赖猴双侧伏隔核后多巴胺D3受体表达明显减少,推测多巴胺D3受体在吗啡成瘾中发挥调节作用.Objective To establish the experimental model of morphine dependent rhesus monkey and observe the changes of D3R expression in nucleus accumbens, hippocampus, cingulate gyrus and cortex of frontal lobe. Methods Four rhesus monkeys were divided into three groups： treatment group （Group A with two monkey） , control group （Group B with one monkey） and normal group （Group C with one monkey） . The model of morphine dependent monkey was established with imitated Seveers＇ method and treated by gamma-knife. The changes of D3R expression in nucleus accumbens, hippocampus, cingulate gyrus and cortex of frontal lobe of three groups were observed using immunohistochemistry. Results The D3R expression in Group B was higher than that in Group C （P 〈 0.001） , and the expression density increased range from 254% to 499%. The D3R expression in Group A was lower than that in Group B （P 〈 0.001） , but higher than that in Group C. The D3R expression in the nucleus accumbens, hippocampus and cingulategyurs decreased significantly and the expression in nucleus accumbens approached normal after gamma-knife treatment. Conclusion The expression of D3R in the nucleus accumbens significantly decreased after gamma-knife treatment, suggesting D3R is effective to adjust the process of morphine addiction.

关 键 词：恒河猴 吗啡依赖 伽玛刀 伏隔核 

分 类 号：R651[医药卫生—外科学]