微生物来源的胆固醇酰基转移酶抑制剂研究 Ⅱ.NA-209A,B的理化特征和结构鉴别  

Studies on acyl-CoA: cholesterol acyltransferase inhibitors from microbial sourcesⅡ. Physico-chemical properties and structure elucidation of NA-209 compounds

作　　者：唐勇[1] 杨大军[1] 姚天爵[1] 

机构地区：[1]中国医学科学院中国协和医科大学医药生物技术研究所,北京100050

出　　处：《中国抗生素杂志》2000年第4期257-259,271,共4页Chinese Journal of Antibiotics

基　　金：国家自然科学基金!(3930 0 1 68);中国医学科学院院校长基金

摘　　要：从曲霉 H717的发酵产物中分离得到两个脂酰辅酶 A:胆固醇酰基转移酶 (ACAT)的抑制剂。根据 EI- MS、FAB- MS和 HREI- MS数据 ,确定其分子式分别为 C2 7H33N3O7(分子量 :5 11.2 )和 C2 7H33N3O5 (分子量 :479.2 )。综合紫外光谱、质谱、核磁共振光谱和 X-结晶衍射等数据解析 ,确定其结构为含有吲哚环的二酮哌嗪类化合物。与文献对照 ,其中 NA- 2 0 9A为 verruculogen的立体异构体 ,而 NA- 2 0 9B则为 fumitremorginB的立体异构体。Two acyl CoA: cholesterol acyltransferase (ACAT) inhibitors, termed NA 209A and B, were isolated from the fermentation broth of Aspergillus sp. H717. Based on EI MS, FAB MS and HREI MS, the formula were determined to be C 27 H 33 N 3O 7 (MW: 511.2) and C 27 H 33 N 3O 5 (MW: 479.2). By the analyses of UV, MS, NMR and single crystal X ray, the structure of NA 209 compounds were elucidated and were identified with the stereo isomers of verruculogen and fumitremorgin B, respectively.

关 键 词：胆固醇酰基转移酶抑制剂 X-衍射 光谱分析 

分 类 号：R978.1[医药卫生—药品] TQ465.92[医药卫生—药学]