高效液相色谱法测定克痨宁胶囊中黄芩苷的含量  

Determination of Baicalin in the Capsule of Kelaoning by HPLC

作　　者：丁野[1] 张嘉乐[1] 

机构地区：[1]湖南省药品检验所,长沙410001

出　　处：《中国药师》2000年第4期225-225,共1页China Pharmacist

摘　　要：目的:测定克痨宁胶囊中黄芩苷的含量。方法:高效液相色谱法。Spherisorb-C_(18)柱,甲醇-水-磷酸(43∶57∶0.2)为流动相,检测波长为280nm。结果:克痨宁胶囊中黄芩苷与多种杂质能有效分离。黄芩苷在0.1002～1.002μg线性关系良好,r=0.9999,平均回收率为101.2％,RSD为1.1％。结论:本方法简便,快速,准确。Objective: To determine the content of Baicalin in Kelaoning Capsule. Method; HPLC was used. Chro-matographic conditions included Spherisorb C18(150mm×4. 6mm) column and the mobile phase waa methonal-water-phosphoric acid(43:57:0.2). The UV detecting wavelength was at 280nm. Results: A satisfactory seperation between Baicalin and impurities was obtained. The calibration curves was linear in the range of 0.1002-1.002μg for Baicalin. The average recovery was 101. 2% (RSD = 1.11%). Conclusion: This method is simple, quick and accurative.

关 键 词：胶囊 克痨宁 黄芩苷 高效液相色谱法 中药 

分 类 号：R286.0[医药卫生—中药学]