正交实验设计优选葎草中黄酮类成分的提取工艺  

Orthogonal Design for Optimizing Extraction Process of Flavonoids from Humulus Scandens

作　　者：孙希孟[1] 楚红英[2] 尹子辰 

机构地区：[1]郑州大学化学与分子工程学院,河南郑州450052 [2]黄河水利职业技术学院环境与化学工程系,河南开封475004

出　　处：《河南化工》2013年第11期33-35,共3页Henan Chemical Industry

摘　　要：以葎草中黄酮类成分提取率为判定提取工艺指标,以分光光度法测定其含量,探讨提取方法、提取溶剂、提取时间3因素3水平正交实验法研究从葎草中提取黄酮类成分的最佳工艺条件。结果表明,最佳提取条件的主次顺序为提取工艺>提取溶剂>提取时间。另外,葎草中不同部位总黄酮含量的大小顺序依次为花>叶>茎。Extracting process of total flavones from humulus scandens is studied. The extraction rate of fla- vonoids is used to evaluate the value standard and the content of flavonoids is determined by ultraviolet - visible spectrophotometry. The optimum extraction process is selected with the orthogonal design of three factors and three levels. The extraction method, extraction solvent and extraction time are the 3 factors that would affect the extraction rate of flavonoids. The results revealed that the effects of all factors on extrac- tion rate from bid to small in order is extraction method 〉 extracting solution 〉 extraction time. In addi- tion, the total content of flavonoids in different part of humulus scandens from bid to small in order was flower 〉 leaf 〉 stem.

关 键 词：葎草 黄酮类化合物 分光光度法 提取 

分 类 号：TQ463.24[化学工程—制药化工]