Isolation Technology of Single Spore and Optimization of Conidia Culture Condition for Ustilaginoidea virens  

作　　者：Haiyong HE Qingqun TAN Xiaojun CHEN Xuehui YANG Shiping WU Lishuang WANG Wongkaew Sopone Jie YUAN 

机构地区：[1]Guizhou Institute of Plant Protection [2]School of Crop Production Technology,Institute of Agricultural Technology,Suranaree University of Technology,Nakon 30000,Thailand

出　　处：《Plant Diseases and Pests》2012年第5期21-23,共3页植物病虫害研究（英文版）

基　　金：Science and Technology Project of Guizhou Province[QKH Major Project( 2012 ) 6012 ];Supported by Science and Technology Fund of Guizhou Province ( QKH J[2009]2103);Science and Technology Research Programof Guizhou Province ( QKH NY[2012]3031);Project of Guizhou Academy of Agricultural Sciences ( [2010]033 and QNKH[Major]07016)

摘　　要：The single spores were isolated from chlamydospores of Ustilaginoidea virens with three different maturities by PSA. The isolated single spores were cul- tured on different media at different temperatures under natural light for inducing conidia to explore the optimum isolation technique of single spore and optimum cul- ture condition of conidia. The results showed that the successful isolating rate of single spore from yellow rice false smut balls reached 90.00%. The sporulatina quantities of isolated single spores cultured on PSD and PDB media at 22 -29 ~C （variable temperature under natural light） or 28 ℃ （constant temperature under dark condition） for 12 d were up to 6.3× 107 and 1.1× 106 spore/mL, respectively. PSA was the most effective method to isolate single spores from yellow rice false smut balls of U. virens. The optimum conidia culture condition included PSD or PDB medium, 22 -29℃ or 28℃, natural light and vibration culture. Key words Ustilaginoidea virens; Single spore isolation; Conidia; Culture conditionThe single spores were isolated from chlamydospores of Ustilaginoidea virens with three different maturities by PSA. The isolated single spores were cul- tured on different media at different temperatures under natural light for inducing conidia to explore the optimum isolation technique of single spore and optimum cul- ture condition of conidia. The results showed that the successful isolating rate of single spore from yellow rice false smut balls reached 90.00%. The sporulatina quantities of isolated single spores cultured on PSD and PDB media at 22 -29 ~C （variable temperature under natural light） or 28 ℃ （constant temperature under dark condition） for 12 d were up to 6.3× 107 and 1.1× 106 spore/mL, respectively. PSA was the most effective method to isolate single spores from yellow rice false smut balls of U. virens. The optimum conidia culture condition included PSD or PDB medium, 22 -29℃ or 28℃, natural light and vibration culture. Key words Ustilaginoidea virens; Single spore isolation; Conidia; Culture condition

关 键 词：Ustilaginoidea virens Single spore isolation CONIDIA Culture condition 

分 类 号：S435.111.4[农业科学—农业昆虫与害虫防治]