吉林省地区朝鲜族和汉族健康人群醛固酮合酶基因(CYP11B2)-344T/C多态性分布的研究  

Study on (CYP11B2)-344T/C gene polymorphism in chinese korea and chinese han healthy people in jilin province

作　　者：刘行[1,2] 隋明醒[2] 王立平[1] 汤赢[2] 全成实[1] 

机构地区：[1]吉林大学病理生物学教育部重点实验室,吉林长春130021 [2]吉林大学白求恩医学院

出　　处：《中国实验诊断学》2013年第7期1194-1196,共3页Chinese Journal of Laboratory Diagnosis

摘　　要：目的分析我国吉林省地区朝鲜族和汉族健康人群醛固酮合酶基因(CYP11B2)-344T/C多态性的分布。方法应用聚合酶链反应(Polymerase Chain Reaction,PCR)和限制性片段长度多态性(Restriction Fragment LengthPolymorphism,RFLP)技术,对67名朝鲜族和59名汉族健康人群CYP11B2基因多态性进行检测,并比较基因型和等位基因频率的分布特征。结果 67名朝鲜族中CYP11B2基因CC、TC和TT基因型频率分别为9%(6/67)、22%(15/67)和69%(46/67);59名汉族中CC、TC和TT基因型频率分别为19%(11/59)、27%(16/59)和54%(32/59),两者差异无显著性(χ2=3.522;P=0.172)。朝鲜族人群C和T等位基因频率分别为20%和80%;汉族人群分别为32%和68%,两者差异具有显著性(χ2=4.763;P=0.029)。结论吉林省地区朝鲜族和汉族健康人群(CYP11B2)-344T/C基因型分布频率差异无显著性,朝鲜族健康人群T等位基因频率高于汉族。Objective To investigate the distribution of （CYPllB2）-344T/C gene polymorphism of chinese han and chinese korea healthy people in jilin province. Methods The techniques of PCR and RFLP were used to detect the poly- morphism of CYPllB2 gene in 67 Korea and 59 Han healthy people. Results The frequency of CC,TC and TT is 9%, 22% and 69%,respected in Korea,and the frequency of CC,TC and TT is 19%,27% and 54% in Han. There were no remarkable differences of the frequency of genotype of CYPllB2 gene（P= 0. 172）between Korea and Han. But there was a significant difference in the frequency distribution of CYPllB2 alleles （P=0. 029） between Korea and Han. Con- clusion There were no remarkable differences of the frequency of genotype of CYPllB2-gene between korea and han. There was a significant difference in the frequency distribution of CYPllB2 alleles between korea and ban. Frequency distribution of T allele of korea is higher than that of han.

关 键 词：醛固酮合酶基因 多态性 朝鲜族 汉族 

分 类 号：R544.1[医药卫生—心血管疾病]